Complete Genomes of Bacillus coagulans S-lac and Bacillus subtilis TO-A JPC, Two Phylogenetically Distinct Probiotics

• Published in: PloS one Vol. 11; no. 6; p. e0156745
• Main Authors: Khatri, Indu, Sharma, Shailza, Ramya, T N C, Subramanian, Srikrishna
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03-06-2016; Public Library of Science (PLoS)
• Subjects: Adhesion; Antibiotic resistance; Antibiotics; Bacillus; Bacillus coagulans; Bacillus coagulans - genetics; Bacillus subtilis; Bacillus subtilis - genetics; Bacteria; Biology; Biology and Life Sciences; Care and treatment; Cell survival; Cell Wall - microbiology; Complications and side effects; Contig Mapping; Diarrhea; Drug resistance; Drug Resistance, Bacterial; E coli; Enterococcus faecium; Enzymes; Epithelial cells; Escherichia coli; Gastrointestinal diseases; Genes, Bacterial; Genetic aspects; Genome, Bacterial; Genomes; Genomics; Health aspects; Irritable bowel syndrome; Lactobacillus; Lactobacillus - genetics; Likelihood Functions; Medicine and Health Sciences; Nucleic Acid Hybridization; Phylogeny; Probiotics; Proteins; Research and Analysis Methods; Signal transduction; Sporulation; Strains (organisms); Transfer RNA; Viral infections; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0156745

Several spore-forming strains of Bacillus are marketed as probiotics due to their ability to survive harsh gastrointestinal conditions and confer health benefits to the host. We report the complete genomes of two commercially available probiotics, Bacillus coagulans S-lac and Bacillus subtilis TO-A JPC, and compare them with the genomes of other Bacillus and Lactobacillus. The taxonomic position of both organisms was established with a maximum-likelihood tree based on twenty six housekeeping proteins. Analysis of all probiotic strains of Bacillus and Lactobacillus reveal that the essential sporulation proteins are conserved in all Bacillus probiotic strains while they are absent in Lactobacillus spp. We identified various antibiotic resistance, stress-related, and adhesion-related domains in these organisms, which likely provide support in exerting probiotic action by enabling adhesion to host epithelial cells and survival during antibiotic treatment and harsh conditions.