Heterogeneity of mast cells and expression of Annexin A1 protein in a second degree burn model with silver sulfadiazine treatment

• Published in: PloS one Vol. 12; no. 3; p. e0173417
• Main Authors: Souza, Helena Ribeiro, de Azevedo, Lucas Ribeiro, Possebon, Lucas, Costa, Sara de Souza, Iyomasa-Pilon, Melina Mizusaki, Oliani, Sonia Maria, Girol, Ana Paula
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09-03-2017; Public Library of Science (PLoS)
• Subjects: A1 protein; Albinism; Animals; Annexin A1 - metabolism; Annexins; Antibiotics; Apoptosis; Biology; Biology and Life Sciences; Burning; Burns; Burns (injuries); Burns - drug therapy; Burns - immunology; Burns - metabolism; Burns - physiopathology; Care and treatment; Cell Count; Cell growth; Cell Proliferation - drug effects; Chymase; Cytokines; Cytokines - metabolism; Cytoplasm - drug effects; Cytoplasm - metabolism; Dermis - drug effects; Dermis - pathology; Disease Models, Animal; Dosage and administration; Epidermis - drug effects; Epidermis - pathology; Fibroblasts; Gene Expression Regulation - drug effects; Healing; Health aspects; Histamine - metabolism; Immunoreactivity; Inflammation; Interleukin 10; Interleukin 6; Laboratories; Macrophages; Macrophages - cytology; Macrophages - drug effects; Mast cells; Mast Cells - cytology; Mast Cells - drug effects; Medicine and Health Sciences; Monocyte chemoattractant protein 1; Peptides; Phases; Physical Sciences; Prevention; Proteins; Rats; Rats, Wistar; Repair; Rodents; Silver sulfadiazine; Silver Sulfadiazine - pharmacology; Silver Sulfadiazine - therapeutic use; Skin; Stem cells; Studies; Sulfadiazine; Therapeutic applications; Tryptase; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Wound healing; Wound Healing - drug effects
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0173417

Mast cells (MCs) participate in all stages of skin healing and one of their mediators is the Annexin A1 protein (AnxA1), linked to inflammation, proliferation, migration and apoptosis processes, but not studied in thermal burns yet. Therefore, our objectives were to evaluate the behavior of MCs and AnxA1 in a second degree burn model, treated or not with silver sulfadiazine 1% (SDP 1%) and associated to macrophages quantification and cytokines dosages. MCs counts showed few cells in the early stages of repair but increased MCs in the final phases in the untreated group. The normal skin presented numerous tryptase-positive MCs that were reduced after burning in all analyzed periods. Differently, few chymase-positive MCs were observed in the early stages of healing, however, increased chymase-positive MCs were found at the final phase in the untreated group. MCs also showed high immunoreactivity for AnxA1 on day 3 in both groups. In the tissue there was a strong protein expression in the early stages of healing, but in the final phases only in the SDP treated animals. TNF-α, IL-1β, IL-6, IL-10 and MCP-1 levels and macrophages quantification were increased in inflammation and reepithelialization phases. Reduced IL-1β, IL-6 and IL-10 levels and numerous macrophages occurred in the treated animals during tissue repair. Our results indicate modulation in the profile of MCs and AnxA1expression during healing by the treatment with SDP 1%, pointing them as targets for therapeutic interventions on skin burns.