Transcriptomics comparison between porcine adipose and bone marrow mesenchymal stem cells during in vitro osteogenic and adipogenic differentiation

• Published in: PloS one Vol. 7; no. 3; p. e32481
• Main Authors: Monaco, Elisa, Bionaz, Massimo, Rodriguez-Zas, Sandra, Hurley, Walter L, Wheeler, Matthew B
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07-03-2012; Public Library of Science (PLoS)
• Subjects: Adipogenesis; Adipogenesis - genetics; Adipose tissue; Adipose Tissue - cytology; Adipose Tissue - metabolism; Algorithms; Angiogenesis; Animal sciences; Animals; Biocompatibility; Bioinformatics; Biology; Biomarkers; Biomedical materials; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Cell cycle; Cell fate; Cell growth; Cell proliferation; Cytoskeleton; Data processing; Differentiation; DNA microarrays; Engineering; Femur; Functional analysis; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Regulatory Networks; Genes; Genomes; Genomics; Guanine nucleotide-binding protein; Immunity; Laboratories; Lipid metabolism; Liver; Male; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Metabolism; Morphology; Osteogenesis; Osteogenesis - genetics; Peroxisome proliferator-activated receptors; Physiological aspects; Proteins; Regeneration; Ribonucleic acid; RNA; Signal Transduction; Signaling; Stem cell transplantation; Stem cells; Studies; Swine; Therapeutic applications; Tissue engineering; Transcriptome - genetics; Tumorigenesis; United States > US; Illinois
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0032481

Bone-marrow mesenchymal stem cells (BMSC) are considered the gold standard for use in tissue regeneration among mesenchymal stem cells (MSC). The abundance and ease of harvest make the adipose-derived stem cells (ASC) an attractive alternative to BMSC. The aim of the present study was to compare the transcriptome of ASC and BMSC, respectively isolated from subcutaneous adipose tissue and femur of 3 adult pigs, during in vitro osteogenic and adipogenic differentiation for up to four weeks. At 0, 2, 7, and 21 days of differentiation RNA was extracted for microarray analysis. A False Discovery Rate ≤0.05 for overall interactions effect and P<0.001 between comparisons were used to determine differentially expressed genes (DEG). Ingenuity Pathway Analysis and DAVID performed the functional analysis of the DEG. Functional analysis of highest expressed genes in MSC and genes more expressed in MSC vs. fully differentiated tissues indicated low immunity and high angiogenic capacity. Only 64 genes were differentially expressed between ASC and BMSC before differentiation. The functional analysis uncovered a potential larger angiogenic, osteogenic, migration, and neurogenic capacity in BMSC and myogenic capacity in ASC. Less than 200 DEG were uncovered between ASC and BMSC during differentiation. Functional analysis also revealed an overall greater lipid metabolism in ASC, while BMSC had a greater cell growth and proliferation. The time course transcriptomic comparison between differentiation types uncovered <500 DEG necessary to determine cell fate. The functional analysis indicated that osteogenesis had a larger cell proliferation and cytoskeleton organization with a crucial role of G-proteins. Adipogenesis was driven by PPAR signaling and had greater angiogenesis, lipid metabolism, migration, and tumorigenesis capacity. Overall the data indicated that the transcriptome of the two MSC is relatively similar across the conditions studied. In addition, functional analysis data might indicate differences in therapeutic application.