Matrix metalloproteinase and tissue inhibitor of metalloproteinase in serum and synovial fluid of osteoarthritic dogs

• Published in: Veterinary and comparative orthopaedics and traumatology Vol. 19; no. 1; p. 49
• Main Authors: Salinardi, B J, Roush, J K, Schermerhorn, T, Mitchell, K E
• Format: Journal Article
• Language: English
• Published: Germany 2006
• Subjects: Animals; Blotting, Western - veterinary; Case-Control Studies; Dog Diseases - blood; Dog Diseases - enzymology; Dog Diseases - metabolism; Dogs; Female; Interferon-alpha - analysis; Male; Metalloproteases - analysis; Osteoarthritis - blood; Osteoarthritis - enzymology; Osteoarthritis - metabolism; Osteoarthritis - veterinary; Synovial Fluid - chemistry; Synovial Fluid - enzymology; Synovial Fluid - metabolism; Tissue Inhibitor of Metalloproteinase-2 - analysis
• ISSN: 0932-0814
• DOI: 10.1055/s-0038-1632973

To better understand the mechanisms responsible for the pathological processes of osteoarthritis (OA) and to potentially identify a profile of changes that could be predictive of early OA, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in the synovial fluid and serum of normal and osteoarthritic dogs were examined. The concentration of MMP-1 in the synovial fluid of osteoarthritic dogs (0.62 +/- 0.16), as measured by densitometry, was significantly higher than that found in control dogs (0.42 +/- 0.19) (P = 0.03). The concentration of MMP-1 in the serum of osteoarthritic dogs (0.74 +/- 0.16) was significantly less than that found in control dogs (0.87 +/- 0.08) (P = 0.05). The concentration of TIMP-2 in the synovial fluid of osteoarthritic dogs (46.2 +/- 21.9 ng/ml) was significantly less than that of control dogs (122.0 +/- 66.5 ng/ml) (P = 0.009). The concentration of TIMP-2 in the serum of osteoarthritic dogs (116.2 +/- 43.1 ng/ml) was not significantly different than that of control dogs (95.1 +/- 94.4 ng/ml) (P = 0.554). In addition, a phospho-tyrosine immunoprecipitation and mass spectrometry were used to isolate and identify interferon-alpha in canine synovial fluid.