Microarray Analysis of Drosophila Development During Metamorphosis

• Published in: Science (American Association for the Advancement of Science) Vol. 286; no. 5447; pp. 2179 - 2184
• Main Authors: White, Kevin P., Rifkin, Scott A., Hurban, Patrick, Hogness, David S.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 10-12-1999; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Anatomy; Animals; Apoptosis; Biological and medical sciences; Cell Differentiation; Cellular biology; Central nervous system; Central Nervous System - cytology; Central Nervous System - growth & development; Classical genetics, quantitative genetics, hybrids; Deoxyribonucleic acid; DNA; Drosophila; Drosophila melanogaster - genetics; Drosophila melanogaster - growth & development; Drosophila melanogaster - metabolism; Ecdysone; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Expression Profiling; Gene Expression Regulation; Genes; Genes, Insect; Genetics of eukaryotes. Biological and molecular evolution; Individualized Instruction; Insect larvae; Insects; Invertebrata; Larva - genetics; Larva - growth & development; Larvae; Larval development; Metamorphosis; Metamorphosis, Biological - genetics; Muscle Development; Muscles - cytology; Neurons; Oligonucleotide Array Sequence Analysis; United States; Insecta; Gene expression; High density oligonucleotide array; Drosophilidae; Regulation(control); Gene; Arthropoda; Development; Glycolysis; Invertebrata; Genome; Drosophila melanogaster; Metamorphosis; Diptera
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.286.5447.2179

Metamorphosis is an integrated set of developmental processes controlled by a transcriptional hierarchy that coordinates the action of hundreds of genes. In order to identify and analyze the expression of these genes, high-density DNA microarrays containing several thousand Drosophila melanogaster gene sequences were constructed. Many differentially expressed genes can be assigned to developmental pathways known to be active during metamorphosis, whereas others can be assigned to pathways not previously associated with metamorphosis. Additionally, many genes of unknown function were identified that may be involved in the control and execution of metamorphosis. The utility of this genome-based approach is demonstrated for studying a set of complex biological processes in a multicellular organism.