Background data of 2-year-old male and female F344 gpt delta rats

• Published in: Journal of Toxicologic Pathology Vol. 34; no. 1; pp. 23 - 31
• Main Authors: Matsushita, Kohei, Ishii, Yuji, Kijima, Aki, Takasu, Shinji, Kuroda, Ken, Takagi, Hisayoshi, Nohmi, Takehiko, Ogawa, Kumiko, Umemura, Takashi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY 01-01-2021; The Japanese Society of Toxicologic Pathology; Japan Science and Technology Agency; Japanese Society of Toxicologic Pathology
• Subjects: background data; Biocompatibility; Carcinogenesis; Carcinogenicity; Carcinogens; F344 rat; Genotypes; gptdelta rat; Lesions; Leukemia; Lymphatic leukemia; Mutation; Nephropathy; Original; Pheochromocytoma; Reporter gene; Spleen; Survival; Toxicity; Transfection; Tumors; background data; gptdelta rat; F344 rat
• ISSN: 0914-9198; 1881-915X; 1347-7404
• DOI: 10.1293/tox.2020-0060

Although gpt delta rats, as reporter gene-transgenic rats, were originally developed for in vivo mutation assays, they have also been used to evaluate chemical carcinogenesis and comprehensive toxicity. Therefore, it is necessary to accumulate background data on carcinogenicity and general toxicity in gpt delta rats. Here, we investigated the background data of 110-week-old male and female F344 gpt delta rats and wild-type rats. There was no effect of reporter gene transfection on animal survival rates and body weights during the experiment. The relative weight of male gpt delta rat adrenals was significantly higher than that of wild-type rats, possibly due to the higher incidence of pheochromocytoma. There were no intergenotype differences in the incidence of nonneoplastic lesions in both sexes, including chronic progressive nephropathy and focus of cellular alteration in the liver, which had a higher incidence in both genotypes. Additionally, the significantly higher incidence of adrenal pheochromocytoma in male gpt delta rats than that in wild-type rats was likely incidental because of the lack of differences in the incidences of preneoplastic (male and female) and neoplastic (female) adrenal lesions in both genotypes. Other neoplastic lesions in both sexes showed no intergenotype differences in incidence rates, although large granular lymphocytic leukemia in the spleen and Leydig cell tumors in the testes of males showed higher incidence rates. Overall, there were no effects of reporter gene transfection on the spectrum of spontaneous lesions in F344 gpt delta rats, thus supporting their applicability in evaluating chemical toxicity and carcinogenicity.