Role of the cryIVD polypeptide in the overall toxicity of Bacillus thuringiensis subsp. israelensis

• Published in: Applied and Environmental Microbiology Vol. 59; no. 11; pp. 3928 - 3930
• Main Authors: Poncet, S, Anello, G, Delecluse, A, Klier, A, Rapoport, G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-11-1993
• Subjects: Aedes; AEDES AEGYPTI; Animals; ANOPHELES STEPHENSI; BACILLUS THURINGIENSIS; Bacillus thuringiensis - genetics; Bacillus thuringiensis - pathogenicity; Bacillus thuringiensis israelensis; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - toxicity; Bacterial Toxins - genetics; Bacterial Toxins - toxicity; Bacteriology; Biochemistry; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; CITOPLASMA; Culex; CULEX PIPIENS; CYTOPLASME; Endotoxins; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Bacterial; Genetics; Hemolysin Proteins; LARVAS; LARVE; Life Sciences; Miscellaneous; Mission oriented research; PEPTIDE; PEPTIDOS; Pest Control, Biological; Plasmids; Proteins; RECOMBINACION; RECOMBINAISON; Recombinant Proteins - genetics; Recombinant Proteins - toxicity; Restriction Mapping; TOXICIDAD; TOXICITE; TOXINAS; TOXINE; Bacillus thuringiensis var. israelensis; Toxicity; Insecta; Culex pipiens; Bacillaceae; Gene expression; Plasmid; Toxin; Anopheles stephensi; Bacillales; Larva; Arthropoda; Culicidae; Deletion; Bacteria; Aedes aegypti; Invertebrata; Diptera; Microbial insecticide
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.59.11.3928-3930.1993

The gene encoding the CryIVD protein of B. thuringiensis subsp. israelensis crystals was disrupted by in vivo recombination. The toxicity of the CryIVD protein-free inclusions was similar to that of the wild-type crystals on Anopheles stephensi larvae but was half the wild-type toxicity on Culex pipiens and Aedes aegypti larvae