Relation between microRNA expression and progression and prognosis of gastric cancer: a microRNA expression analysis

• Published in: The lancet oncology Vol. 11; no. 2; pp. 136 - 146
• Main Authors: Ueda, Tetsuya, MD, Volinia, Stefano, PhD, Okumura, Hiroshi, MD, Shimizu, Masayoshi, BS, Taccioli, Cristian, PhD, Rossi, Simona, PhD, Alder, Hansjuerg, PhD, Liu, Chang-gong, Prof, Oue, Naohide, MD, Yasui, Wataru, Prof, Yoshida, Kazuhiro, Prof, Sasaki, Hiroki, PhD, Nomura, Sachiyo, MD, Seto, Yasuyuki, Prof, Kaminishi, Michio, Prof, Calin, George A, MD, Croce, Carlo M, Prof
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-02-2010; Elsevier Limited
• Subjects: Aged; Cluster analysis; Disease; Disease Progression; Female; Gastric cancer; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genes; Genomes; Hematology; Hematology, Oncology and Palliative Medicine; Humans; Male; Medical prognosis; Metastasis; MicroRNAs; MicroRNAs - genetics; Middle Aged; Oligonucleotide Array Sequence Analysis; Patients; Prognosis; Regression analysis; Statistical analysis; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Survival analysis; Tumorigenesis; Tumors; United Kingdom > UK; United States > US
• ISSN: 1470-2045; 1474-5488
• DOI: 10.1016/S1470-2045(09)70343-2

Summary Background Analyses of microRNA expression profiles have shown that many microRNAs are expressed aberrantly and correlate with tumorigenesis, progression, and prognosis of various haematological and solid tumours. We aimed to assess the relation between microRNA expression and progression and prognosis of gastric cancer. Methods 353 gastric samples from two independent subsets of patients from Japan were analysed by microRNA microarray. MicroRNA expression patterns were compared between non-tumour mucosa and cancer samples, graded by diffuse and intestinal histological types and by progression-related factors (eg, depth of invasion, metastasis, and stage). Disease outcome was calculated by multivariable regression analysis to establish whether microRNAs are independent prognostic factors. Findings In 160 paired samples of non-tumour mucosa and cancer, 22 microRNAs were upregulated and 13 were downregulated in gastric cancer; 292 (83%) samples were distinguished correctly by this signature. The two histological subtypes of gastric cancer showed different microRNA signatures: eight microRNAs were upregulated in diffuse-type and four in intestinal-type cancer. In the progression-related signature, miR-125b, miR-199a, and miR-100 were the most important microRNAs involved. Low expression of let-7g (hazard ratio 2·6 [95% CI 1·3–4·9]) and miR-433 (2·1 [1·1–3·9]) and high expression of miR-214 (2·4 [1·2–4·5]) were associated with unfavourable outcome in overall survival independent of clinical covariates, including depth of invasion, lymph-node metastasis, and stage. Interpretation MicroRNAs are expressed differentially in gastric cancers, and histological subtypes are characterised by specific microRNA signatures. Unique microRNAs are associated with progression and prognosis of gastric cancer. Funding National Cancer Institute.