A prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting

• Published in: BMC infectious diseases Vol. 16; no. 1; p. 705
• Main Authors: Martínez-Vega, Ruth Aralí, Rodriguez-Morales, Alfonso J, Bracho-Churio, Yalil Tomás, Castro-Salas, Mirley Enith, Galvis-Ovallos, Fredy, Díaz-Quijano, Ronald Giovanny, Luna-González, María Lucrecia, Castellanos, Jaime E, Ramos-Castañeda, José, Diaz-Quijano, Fredi Alexander
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25-11-2016; BioMed Central; BMC
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Care and treatment; Child; Child, Preschool; Clinical Protocols; Colombia - epidemiology; Complications and side effects; Cross-Sectional Studies; Dengue; Dengue - diagnosis; Dengue - economics; Dengue - epidemiology; Dengue - prevention & control; Dengue Vaccines - economics; Enzyme-Linked Immunosorbent Assay; Female; Follow-Up Studies; Health aspects; Health Knowledge, Attitudes, Practice; Health Services Needs and Demand - economics; Health Services Needs and Demand - statistics & numerical data; Humans; Immunoglobulin G; Incidence; Infant; Infant, Newborn; Infectious diseases; Influence; Knowledge; Male; Middle Aged; Prospective Studies; Risk Factors; Seroepidemiologic Studies; Seroprevalence; Study Protocol; Vaccines; Young Adult; Colombia; Colombia; Dengue; Vaccines; Knowledge; Risk factors; Incidence; Seroprevalence
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-016-2055-4

Dengue is one of the most important vector-borne diseases in the world, causing significant morbidity and economic impact. In Colombia, dengue is a major public health problem. Departments of La Guajira, Cesar and Magdalena are dengue endemic areas. The objective of this research is to determine the seroprevalence and the incidence of dengue virus infection in the participating municipalities from these Departments, and also establish the association between individual and housing factors and vector indices with seroprevalence and incidence. We will also assess knowledge, attitudes and practices, and willingness-to-pay for dengue vaccine.
A cohort study will be assembled with a clustered multistage sampling in 11 endemic municipalities. Approximately 1000 homes will be visited to enroll people older than one year who living in these areas, who will be followed for 1 year. Dengue virus infections will be evaluated using IgG indirect ELISA and IgM and IgG capture ELISA. Additionally, vector indices will be measured, and adult mosquitoes will be captured with aspirators. Ovitraps will be used for continuous estimation of vector density.
This research will generate necessary knowledge to design and implement strategies with a multidimensional approach that reduce dengue morbidity and mortality in La Guajira and other departments from Colombian Caribbean.