Francisella tularensis: No Evidence for Transovarial Transmission in the Tularemia Tick Vectors Dermacentor reticulatus and Ixodes ricinus

• Published in: PloS one Vol. 10; no. 8; p. e0133593
• Main Authors: Genchi, Marco, Prati, Paola, Vicari, Nadia, Manfredini, Andrea, Sacchi, Luciano, Clementi, Emanuela, Bandi, Claudio, Epis, Sara, Fabbi, Massimo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05-08-2015; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Arachnid Vectors - microbiology; Arachnids; Arthropods; Bioassays; Biology; Bioterrorism; Bites; Care and treatment; Complications and side effects; Contamination; Culicidae; Degeneration; Dermacentor - microbiology; Diagnosis; Disease transmission; DNA, Bacterial - genetics; Eggs; Electron microscopy; Epidemiology; Female; Females; Fluorescence; Fluorescence in situ hybridization; Francisella tularensis; Francisella tularensis - genetics; Francisella tularensis - physiology; Francisella tularensis - ultrastructure; Guinea Pigs; Hard ticks; Health aspects; Host-Pathogen Interactions; Humans; In Situ Hybridization, Fluorescence; Infections; Insect bites; Ixodes - microbiology; Ixodes ricinus; Laboratory animals; Larvae; Mammals; Microscopy, Electron, Transmission; Morbidity; Mortality; Niches; Oocytes; Oocytes - microbiology; Ovary - microbiology; Polymerase Chain Reaction; Public health; Reptiles & amphibians; Risk factors; RNA, Ribosomal, 23S - genetics; Rodents; Studies; Ticks; Transmission electron microscopy; Tularemia; Tularemia - diagnosis; Tularemia - microbiology; Tularemia - transmission; Vectors; Zoonoses; United States > US; Italy; North America
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0133593

Tularemia is a zoonosis caused by the Francisella tularensis, a highly infectious Gram-negative coccobacillus. Due to easy dissemination, multiple routes of infection, high environmental contamination and morbidity and mortality rates, Francisella is considered a potential bioterrorism threat and classified as a category A select agent by the CDC. Tick bites are among the most prevalent modes of transmission, and ticks have been indicated as a possible reservoir, although their reservoir competence has yet to be defined. Tick-borne transmission of F. tularensis was recognized in 1923, and transstadial transmission has been demonstrated in several tick species. Studies on transovarial transmission, however, have reported conflicting results.
The aim of this study was to evaluate the role of ticks as reservoirs for Francisella, assessing the transovarial transmission of F. tularensis subsp. holarctica in ticks, using experimentally-infected females of Dermacentor reticulatus and Ixodes ricinus.
Transmission electron microscopy and fluorescence in situ hybridization showed F. tularensis within oocytes. However, cultures and bioassays of eggs and larvae were negative; in addition, microscopy techniques revealed bacterial degeneration/death in the oocytes.
These results suggest that bacterial death might occur in oocytes, preventing the transovarial transmission of Francisella. We can speculate that Francisella does not have a defined reservoir, but that rather various biological niches (e.g. ticks, rodents), that allow the bacterium to persist in the environment. Our results, suggesting that ticks are not competent for the bacterium vertical transmission, are congruent with this view.