Externally controlled triggered-release of drug from PLGA micro and nanoparticles

• Published in: PloS one Vol. 9; no. 12; p. e114271
• Main Authors: Hua, Xin, Tan, Shengnan, Bandara, H M H N, Fu, Yujie, Liu, Siguo, Smyth, Hugh D C
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05-12-2014; Public Library of Science (PLoS)
• Subjects: Acids; Antibacterial activity; Antibiotics; Antimicrobial activity; Biofilms; Biofilms - drug effects; Cancer therapies; Ciprofloxacin; Ciprofloxacin - administration & dosage; Ciprofloxacin - chemistry; Controlled release; Cystic fibrosis; Delayed-Action Preparations - administration & dosage; Drug Delivery Systems; Encapsulation; Fever; Glycolic acid; Health aspects; Humans; Hyperthermia; Infection; Laboratories; Lactic Acid - administration & dosage; Lactic Acid - chemistry; Magnetic fields; Magnetite Nanoparticles - administration & dosage; Magnetite Nanoparticles - chemistry; Medicine and Health Sciences; Nanoparticles; Particle Size; Pharmacy; Polyglycolic Acid - administration & dosage; Polyglycolic Acid - chemistry; Polylactic Acid-Polyglycolic Acid Copolymer; Polylactide-co-glycolide; Polymers; Polyvinyl alcohol; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - pathogenicity; Pseudomonas Infections - drug therapy; Pseudomonas Infections - microbiology; Stability; Sustained release; Urinary tract diseases; Urinary tract infections; Urogenital system; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0114271

Biofilm infections are extremely hard to eradicate and controlled, triggered and controlled drug release properties may prolong drug release time. In this study, the ability to externally control drug release from micro and nanoparticles was investigated. We prepared micro/nanoparticles containing ciprofloxacin (CIP) and magnetic nanoparticles encapsulated in poly (lactic-co-glycolic acid) PLGA. Both micro/nanoparticles were observed to have narrow size distributions. We investigated and compared their passive and externally triggered drug release properties based on their different encapsulation structures for the nano and micro systems. In passive release studies, CIP demonstrated a fast rate of release in first 2 days which then slowed and sustained release for approximately 4 weeks. Significantly, magnetic nanoparticles containing systems all showed ability to have triggered drug release when exposed to an external oscillating magnetic field (OMF). An experiment where the OMF was turned on and off also confirmed the ability to control the drug release in a pulsatile manner. The magnetically triggered release resulted in a 2-fold drug release increase compared with normal passive release. To confirm drug integrity following release, the antibacterial activity of released drug was evaluated in Pseudomonas aeruginosa biofilms in vitro. CIP maintained its antimicrobial activity after encapsulation and triggered release.