Beware the intruder: Real time observation of infiltrated neutrophils and neutrophil-Microglia interaction during stroke in vivo

• Published in: PloS one Vol. 13; no. 3; p. e0193970
• Main Authors: Neumann, Jens, Henneberg, Sophie, von Kenne, Susanne, Nolte, Niklas, Müller, Andreas J, Schraven, Burkhart, Görtler, Michael W, Reymann, Klaus G, Gunzer, Matthias, Riek-Burchardt, Monika
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15-03-2018; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Bone marrow; Brain; Brain research; Development and progression; Experiments; Granulocytes; Health aspects; Immune response; Immune system; Immunology; Infiltration; Inflammation; Ischemia; Leukocytes (granulocytic); Leukocytes (neutrophilic); Medicine and Health Sciences; Microglia; Microscopy; Neurology; Neurosciences; Neutrophils; Parenchyma; Pathogenesis; Physiological aspects; Research and Analysis Methods; Rodents; Stem cells; Stroke; Transgenic animals; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0193970

Inflammation plays an important role in the pathogenesis of ischemic stroke including an acute and prolonged inflammatory process. The role of neutrophil granulocytes as first driver of the immune reaction from the blood site is under debate due to controversial findings. In bone marrow chimeric mice we were able to study the dynamics of tdTomato-expressing neutrophils and GFP-expressing microglia after photothrombosis using intravital two-photon microscopy. We demonstrate the infiltration of neutrophils into the brain parenchyma and confirm a long-lasting contact between neutrophils and microglia as well as an uptake of neutrophils by microglia clearing the brain from peripheral immune cells.