Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes)

• Published in: PloS one Vol. 17; no. 12; p. e0273064
• Main Authors: Watanabe-Asaka, Tomomi, Niihori, Maki, Sonobe, Hiroki, Igarashi, Kento, Oda, Shoji, Iwasaki, Ken-Ichi, Katada, Yoshihiko, Yamashita, Toshikazu, Terada, Masahiro, Baba, Shoji A, Mitani, Hiroshi, Mukai, Chiaki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30-12-2022; Public Library of Science (PLoS)
• Subjects: Adult; Anesthesia; Animals; Atropine; Autonomic Nervous System; Biology and Life Sciences; Cameras; Digital video; EKG; Electrocardiogram; Electrocardiography; Embryonic development; Embryos; Environmental effects; Environmental law; Evaluation; Experiments; Fish; Fishes; Frequency dependence; Frequency ranges; Hatching; Health aspects; Heart beat; Heart diseases; Heart rate; Heart Rate - physiology; Humans; Laboratories; Larvae; Laws, regulations and rules; Measurement; Medicine and Health Sciences; Modulation; Nervous system; Oryzias; Oryzias latipes; Parasympathetic nervous system; Physical Sciences; Physiology; Propranolol; Research and Analysis Methods; Sympathetic Nervous System; Variability; Vertebrates; Zebrafish; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0273064

Small teleosts have recently been established as models of human diseases. However, measuring heart rate by electrocardiography is highly invasive for small fish and not widely used. The physiological nature and function of vertebrate autonomic nervous system (ANS) modulation of the heart has traditionally been investigated in larvae, transparent but with an immature ANS, or in anesthetized adults, whose ANS activity may possibly be disturbed under anesthesia. Here, we defined the frequency characteristics of heart rate variability (HRV) modulated by the ANS from observations of heart movement in high-speed movie images and changes in ANS regulation under environmental stimulation in unanesthetized adult medaka (Oryzias latipes). The HRV was significantly reduced by atropine (1 mM) in the 0.25-0.65 Hz and by propranolol (100 μM) at 0.65-1.25 Hz range, suggesting that HRV in adult medaka is modulated by both the parasympathetic and sympathetic nervous systems within these frequency ranges. Such modulations of HRV by the ANS in adult medaka were remarkably suppressed under anesthesia and continuous exposure to light suppressed HRV only in the 0.25-0.65 Hz range, indicating parasympathetic withdrawal. Furthermore, pre-hatching embryos did not show HRV and the power of HRV developed as fish grew. These results strongly suggest that ANS modulation of the heart in adult medaka is frequency-dependent phenomenon, and that the impact of long-term environmental stimuli on ANS activities, in addition to development of ANS activities, can be precisely evaluated in medaka using the presented method.