K-13 propeller gene polymorphisms isolated between 2014 and 2017 from Cameroonian Plasmodium falciparum malaria patients

• Published in: PloS one Vol. 14; no. 9; p. e0221895
• Main Authors: Eboumbou Moukoko, Carole Else, Huang, Fang, Nsango, Sandrine Eveline, Kojom Foko, Loic Pradel, Ebong, Serge Bruno, Epee Eboumbou, Patricia, Yan, He, Sitchueng, Livia, Garke, Bouba, Ayong, Lawrence
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03-09-2019; Public Library of Science (PLoS)
• Subjects: Alleles; Antimalarials - pharmacology; Antimicrobial agents; Artemisinin; Artemisinins - pharmacology; Biology and Life Sciences; Blood; Cameroon - epidemiology; Child; Child, Preschool; Deoxyribonucleic acid; Disease control; Disease transmission; DNA; Dosage and administration; Drug resistance; Drug Resistance - genetics; EDTA; Epidemiology; Erythrocytes; Female; Genes, Protozoan; Genetic aspects; Genetic polymorphisms; Genetic research; Humans; Kelch Repeat; Littoral environments; Malaria; Malaria, Falciparum - epidemiology; Malaria, Falciparum - parasitology; Male; Medicine and Health Sciences; Mutation; Parasite resistance; Parasites; Parasitic diseases; Patient outcomes; People and Places; Pharmaceutical sciences; Plasmodium falciparum; Plasmodium falciparum - drug effects; Plasmodium falciparum - genetics; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic; Population studies; Prospective Studies; Protozoan Proteins - genetics; Pyrimethamine; Research and analysis methods; Studies; Vector-borne diseases; Cameroon; Southeast Asia; Kenya; China; Africa
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0221895

The emergence of artemisinin-resistant parasites since the late 2000s at the border of Cambodia and Thailand poses serious threats to malaria control globally, particularly in Africa which bears the highest malaria transmission burden. This study aimed to obtain reliable data on the current state of the kelch13 molecular marker for artemisinin resistance in Plasmodium falciparum in Cameroon. DNA was extracted from the dried blood spots collected from epidemiologically distinct endemic areas in the Center, Littoral and North regions of Cameroon. Nested PCR products from the Kelch13-propeller gene were sequenced and analyzed on an ABI 3730XL automatic sequencer. Of 219 dried blood spots, 175 were sequenced successfully. We identified six K13 mutations in 2.9% (5/175) of samples, including 2 non-synonymous, the V589I allele had been reported in Africa already and one new allele E612K had not been reported yet. These two non-synonymous mutations were uniquely found in parasites from the Littoral region. One sample showed two synonymous mutations within the kelch13 gene. We also observed two infected samples with mixed K13 mutant and K13 wild-type infection. Taken together, our data suggested the circulation of the non-synonymous K13 mutations in Cameroon. Albeit no mutations known to be associated with parasite clearance delays in the study population, there is need for continuous surveillance for earlier detection of resistance as long as ACTs are used and scaled up in the community.