Metabonomic Evaluation of Chronic Unpredictable Mild Stress-Induced Changes in Rats by Intervention of Fluoxetine by HILIC-UHPLC/MS

• Published in: PloS one Vol. 10; no. 6; p. e0129146
• Main Authors: Zhao, Longshan, Xiong, Zhili, Lu, Xiumei, Zheng, Shuning, Wang, Fang, Ge, Lin, Su, Guangyue, Yang, Jingyu, Wu, Chunfu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16-06-2015; Public Library of Science (PLoS)
• Subjects: Amino acids; Animals; Antidepressive Agents, Second-Generation - pharmacology; Antidepressive Agents, Second-Generation - therapeutic use; Biomarkers; Biomarkers - blood; Biomarkers - metabolism; Chromatography; Chromatography, High Pressure Liquid - methods; Depression - blood; Depression - drug therapy; Depression - metabolism; Fluoxetine; Fluoxetine - pharmacology; Fluoxetine - therapeutic use; Levocarnitine; Liquid chromatography; Male; Mass Spectrometry - methods; Metabolites; Metabolomics - methods; Rats; Rats, Sprague-Dawley; Stress, Physiological - drug effects; Liaoning China; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0129146

Hydrophilic interaction-ultra high performance liquid chromatography (HILIC-UHPLC) allows the analysis of highly polar metabolites, providing complementary information to reversed-phase (RP) chromatography. By optimization of the preparation and analytical conditions in HILIC mode, HILIC-UHPLC/MS was applied for the global metabolic profiling of rat plasma samples generated in an experimental model of chronic unpredictable mild stress (CUMS), and the concomitant investigation of the protective effect of fluoxetine was also evaluated. Identification of plasma metabolic profiles indicated that significant changes in specific metabolites occurred after fluoxetine exposure, including increased phenylalanine, serine, acetyl-L-carnitine, carnitine and decreased creatine, betaine, proline, tryptophan, tyrosine, C16:0 LPC. Some novel biomarkers from this HILIC-UHPLC/MS approach were betaine, proline, tyrosine creatine and serine compared with the results of RP-UHPLC/MS. The complementary nature of this technique is confirmed and is on agreement with previously published studies.