CCT6A knockdown suppresses osteosarcoma cell growth and Akt pathway activation in vitro

• Published in: PloS one Vol. 17; no. 12; p. e0279851
• Main Authors: Zeng, Weiquan, Wu, Meizhu, Cheng, Ying, Liu, Liya, Han, Yuying, Xie, Qiurong, Li, Jiapeng, Wei, Lihui, Fang, Yi, Chen, Youqin, Peng, Jun, Shen, Aling
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30-12-2022; Public Library of Science (PLoS)
• Subjects: AKT protein; Analysis; Antibodies; Apoptosis; Apoptosis - genetics; Bcl-2 protein; Biology and Life Sciences; Bone cancer; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Cancer therapies; Carbon dioxide; Cell Cycle; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - genetics; Cell survival; Cell viability; Chaperonin Containing TCP-1 - genetics; Chaperonin Containing TCP-1 - metabolism; Cyclin-dependent kinases; Diagnosis; Ethylenediaminetetraacetic acid; G1 Phase; Gene expression; Genetic aspects; Genomic analysis; Genomics; Growth; Humans; Infection; Kinases; Lung cancer; Lymphocytes B; Lymphoma; Lymphomas; Medical prognosis; Metastasis; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma cells; Protein kinases; Proteins; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Research and Analysis Methods; Ribonucleic acid; RNA; Sarcoma; siRNA; Transfection; Visualization (Computers); China; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0279851

We assessed the role of the protein-coding gene chaperonin-containing TCP1 subunit 6A (CCT6A) in osteosarcoma, as this is currently unknown. Using data from the R2 online genomic analysis and visualization application, we found that CCT6A messenger ribonucleic acid (RNA) expression is increased in osteosarcoma tissue and cells. Transfection of CCT6A small interfering RNA into cultured osteosarcoma cells revealed that CCT6A knockdown attenuates cell growth, cell viability, cell survival, and induced apoptosis and cell cycle progression at the G0/G1 phases. Moreover, CCT6A knockdown downregulated phospho-protein kinase B (p-Akt), cyclinD1 and B-cell lymphoma-2, whereas upregulated Bcl-2-associated X-protein expression. Thus, CCT6A knockdown inhibits cell proliferation, induces cell apoptosis, and suppresses the Akt pathway.