The activity-integrated method for quality assessment of reduning injection by on-line DPPH-CE-DAD

• Published in: PloS one Vol. 9; no. 9; p. e106254
• Main Authors: Chang, Yan-xu, Liu, Jiao, Bai, Yang, Li, Jin, Liu, Er-wei, He, Jun, Jiao, Xiu-cheng, Wang, Zhen-zhong, Gao, Xiu-Mei, Zhang, Bo-li, Xiao, Wei
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02-09-2014; Public Library of Science (PLoS)
• Subjects: Acetonitriles - chemistry; Acids; Acupuncture; Analysis; Antioxidants; Antioxidants (Nutrients); Antioxidants - analysis; beta-Cyclodextrins - chemistry; Biphenyl Compounds - chemistry; Buffer solutions; Caffeic acid; Calibration; Capillary electrophoresis; Chinese medicine; Chlorogenic acid; Chromatography; Drugs, Chinese Herbal - analysis; Electricity; Electronics - instrumentation; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Free radicals; Herbal medicine; Hydrogen-Ion Concentration; Ingredients; Inhibitory Concentration 50; Injection; Injections; Laboratories; Limit of Detection; Medicine and Health Sciences; Methods; Online Systems; pH effects; Pharmaceuticals; Pharmacology; Picrates - chemistry; Pneumonia; Quality assessment; Quality control; Quality management; Reagents; Reference Standards; Research and Analysis Methods; Temperature; Test procedures; Time Factors; United States > US; China; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0106254

A sensitive on-line DPPH-CE-DAD method was developed and validated for both screening and determining the concentration of seven antioxidants of Reduning injection. The pH and concentrations of buffer solution, SDS, β-CD and organic modifier were studied for the detection of DPPH and seven antioxidants. By on-line mixing DPPH and sample solution, a DPPH-CE method for testing the antioxidant activity of the complex matrix was successfully established and used to screen the antioxidant components of Reduning injection. Then, antioxidant components including caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid were quantified by the newly established CE-DAD method. Finally, the total antioxidant activity and the multiple active components were selected as markers to evaluate the quality of Reduning injection. The results demonstrated that the on-line DPPH-CE-DAD method was reagent-saving, rapid and feasible for on-line simultaneous determination of total pharmacological activity and contents of multi-components samples. It was also a powerful method for evaluating the quality control and mechanism of action of TCM injection.