Proteome analysis of Phytomonas serpens, a phytoparasite of medical interest

The protozoan Phytomonas serpens (class Kinetoplastea) is an important phytoparasite that has gained medical importance due to its similarities to Trypanosoma cruzi, the etiological agent of Chagas disease. The present work describes the first proteome analysis of P. serpens. The parasite was separa...

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Published in:PloS one Vol. 13; no. 10; p. e0204818
Main Authors: Dos Santos Júnior, Agenor de Castro Moreira, Ricart, Carlos André Ornelas, Pontes, Arthur Henriques, Fontes, Wagner, Souza, Agnelo Rodrigues de, Castro, Mariana Souza, de Sousa, Marcelo Valle, de Lima, Beatriz Dolabela
Format: Journal Article
Language:English
Published: United States Public Library of Science 10-10-2018
Public Library of Science (PLoS)
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Summary:The protozoan Phytomonas serpens (class Kinetoplastea) is an important phytoparasite that has gained medical importance due to its similarities to Trypanosoma cruzi, the etiological agent of Chagas disease. The present work describes the first proteome analysis of P. serpens. The parasite was separated into cytosolic and high density organelle fractions, which, together with total cell extract, were subjected to LC-MS/MS analyses. Protein identification was conducted using a comprehensive database composed of genome sequences of other related kinetoplastids. A total of 1,540 protein groups were identified among the three sample fractions. Sequences from Phytomonas sp. in the database allowed the highest number of identifications, with T. cruzi and T. brucei the human pathogens providing the greatest contribution to the identifications. Based on the proteomics data obtained, we proposed a central metabolic map of P. serpens, which includes all enzymes of the citric acid cycle. Data also revealed a new range of proteins possibly responsible for immunological cross-reactivity between P. serpens and T. cruzi.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0204818