In Situ Transplantation of Alginate Bioencapsulated Adipose Tissues Derived Stem Cells (ADSCs) via Hepatic Injection in a Mouse Model

• Published in: PloS one Vol. 10; no. 9; p. e0138184
• Main Authors: Chen, Mong-Jen, Lu, Yuanqing, Simpson, Nicholas E, Beveridge, Mark J, Elshikha, Ahmed S, Akbar, Mohammad Ahsanul, Tsai, Hsin-Yin, Hinske, Stephanie, Qin, Junling, Grunwitz, Christian R, Chen, Tina, Brantly, Mark L, Song, Sihong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15-09-2015; Public Library of Science (PLoS)
• Subjects: Adipose tissue; Adipose Tissue - cytology; Alginates - chemistry; Alginic acid; alpha 1-Antitrypsin - genetics; Animal tissues; Animals; Biomaterials; Biomedical materials; Bone marrow; Bone marrow transplantation; Capsules; Care and treatment; Cell adhesion & migration; Cell Differentiation; Cell proliferation; Differentiation; Feasibility Studies; Glucuronic Acid - chemistry; Hepatocytes; Hepatocytes - cytology; Hepatology; Hexuronic Acids - chemistry; Humans; Hypoxia; In vivo methods and tests; Injection; Injections; Liver; Liver - cytology; Liver diseases; Liver transplants; Lungs; Male; Medicine; Metabolism; Methods; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microspheres; Organs; Pharmaceutical sciences; Pharmacy; Physiological aspects; Rodents; Spleen; Stem cell transplantation; Stem Cell Transplantation - methods; Stem cells; Stem Cells - chemistry; Stem Cells - cytology; Surgical implants; Tissue engineering; Transgenic animals; Transgenic mice; Transplantation; Transplants & implants; United States; Florida; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0138184

Adipose tissue derived stem cells (ADSCs) transplantation has recently gained widespread enthusiasm, particularly in the perspective to use them as potential alternative cell sources for hepatocytes in cell based therapy, mainly because of their capability of hepatogenic differentiation in vitro and in vivo. But some challenges remain to be addressed, including whether ADSCs can be provided effectively to the target organ and whether subsequent proliferation of transplanted cells can be achieved. To date, intrasplenic injection is the conventional method to deliver ADSCs into the liver; however, a number of donor cells retained in the spleen has been reported. In this study, our objective is to evaluate a novel route to transplant ADSCs specifically to the liver. We aimed to test the feasibility of in situ transplantation of ADSCs by injecting bioencapsulated ADSCs into the liver in mouse model.
The ADSCs isolated from human alpha 1 antitrypsin (M-hAAT) transgenic mice were used to allow delivered ADSCs be readily identified in the liver of recipient mice, and alginate was selected as a cell carrier. We first evaluated whether alginate microspheres are implantable into the liver tissue by injection and whether ADSCs could migrate from alginate microspheres (study one). Once proven, we then examined the in vivo fate of ADSCs loaded microspheres in the liver. Specifically, we evaluated whether transplanted, undifferentiated ASDCs could be induced by the local microenvironment toward hepatogenic differentiation and the distribution of surviving ADSCs in major tissue organs (study two).
Our results indicated ADSCs loaded alginate microspheres were implantable into the liver. Both degraded and residual alginate microspheres were observed in the liver up to three weeks. The viable ADSCs were detectable surrounding degraded and residual alginate microspheres in the liver and other major organs such as bone marrow and the lungs. Importantly, transplanted ADSCs underwent hepatogenic differentiation to become cells expressing albumin in the liver. These findings improve our understanding of the interplay between ADSCs (donor cells), alginate (biomaterial), and local microenvironment in a hepatectomized mouse model, and might improve the strategy of in situ transplantation of ADSCs in treating liver diseases.