Physiological increase of yolk testosterone level does not affect oxidative status and telomere length in gull hatchlings

• Published in: PloS one Vol. 13; no. 10; p. e0206503
• Main Authors: Parolini, Marco, Possenti, Cristina Daniela, Romano, Andrea, Caprioli, Manuela, Rubolini, Diego, Saino, Nicola
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26-10-2018; Public Library of Science (PLoS)
• Subjects: Androgens; Antioxidants; Antioxidants (Nutrients); Bioavailability; Biology and Life Sciences; Birds; Eggs; Embryos; Environmental science; Gulls; Hatching; Hormones; Life history; Life span; Lipid peroxidation; Medicine and Health Sciences; Metabolism; Offspring; Oxidants; Oxidative stress; Oxidizing agents; Phenotypes; Physiological aspects; Physiology; Plasma; Standard deviation; Supplementation; Supplements; Telomerase; Telomeres; Testosterone; Yolk; Italy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0206503

Conditions experienced during early-life can cause the onset of oxidative stress, resulting in pervasive effects on diverse life-history traits, including lifespan. In birds, maternally-transferred egg substances may exert positive or negative influence over the offspring phenotype. Among these, testosterone can upregulate the bioavailability of certain antioxidants but simultaneously promotes the production of pro-oxidants, leading to an oxidative stress situation, which is one of the main forces causing telomere attrition However, no study has investigated the role of this androgen on telomere dynamics in birds and little is known about the effects of yolk testosterone on oxidative status in early-life of these species. We physiologically increased the levels of yolk testosterone by in ovo injections in yellow-legged gull (Larus michahellis) to evaluate the effects induced by this androgen on hatchlings plasma total antioxidant capacity, amount of pro-oxidant molecules and telomere length at hatching. Testosterone supplementation did not increase hatchling body growth, did not result in the overproduction of pro-oxidant molecules nor a reduction of antioxidant capacity. Accordingly, telomere length at hatching was not affected by testosterone treatment, although hatchlings from the third-laid eggs showed shorter telomeres than their siblings from first- and second-laid eggs, independently of testosterone treatment. Our results suggest that injection of physiological levels of testosterone does not induce oxidative stress to hatchlings and, consequently do not affect telomere dynamics during early post-natal periods.