Clinical efficacy and pharmacokinetics of meloxicam in Mediterranean buffalo calves (Bubalus bubalis)

• Published in: PloS one Vol. 12; no. 10; p. e0187252
• Main Authors: Cagnardi, Petra, Guccione, Jacopo, Villa, Roberto, D'Andrea, Luigi, Di Loria, Antonio, Ferrante, Maria Carmela, Borriello, Giuliano, Zicarelli, Luigi, Ciaramella, Paolo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27-10-2017; Public Library of Science (PLoS)
• Subjects: Analgesics; Animal sciences; Animals; Bioavailability; Biology and Life Sciences; Body weight; Bubalus bubalis; Buffaloes; Calves; Cattle; Cortisol; Effectiveness; Food safety; Half-life; Health aspects; Heart rate; Male; Medicine and Health Sciences; Meloxicam; Metabolism; Nonsteroidal anti-inflammatory drugs; Pain; Pharmacokinetics; Pharmacology; Physiological aspects; Respiration; Respiratory rate; Studies; Surgery; Thiazines - pharmacokinetics; Thiazines - therapeutic use; Thiazoles - pharmacokinetics; Thiazoles - therapeutic use; Veterinary medicine; Water buffalo; Zoology; Italy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0187252

The aims of the investigation were to establish for the first time (i) clinical efficacy and (ii) pharmacokinetic profile of meloxicam intravenously (IV) administered in male Mediterranean buffalo calves after surgical orchiectomy. The study was performed on 10 healthy buffalo calves, between 4 and 5 months old and between 127 and 135 kg of body weight (b.w.). An IV injection of 0.5 mg/kg b.w. of meloxicam was administered in six calves (treated group, TG) immediately after surgery; the other four animals were used as untreated control group (CG). The clinical efficacy of meloxicam was evaluated pre- and post-surgery by monitoring respiratory rate (RR), heart rate (HR), rectal temperature (T°C), serum cortisol levels (SCL) and pain score (PS). Significant inter-groups differences were detected at sampling times (T): 4 hour (h) for RR (P<0.05), at T1-4-6-8 h for PS (P<0.05) and at T4-6-8 h for SCL (P < 0.0001). Regarding the mean intra-group values observed pre (T0) and post-surgery (from T15 min to T72 h), significant difference between the groups were found for RR (P<0.01), PS and SCL (P<0.05). The pharmacokinetic profile was best fitted by a two-compartmental model and characterized by a fast distribution half-life and slow elimination half-life (0.09 ± 0.06 h and 21.51 ± 6.4 h, respectively) and meloxicam mean concentrations at 96 h was of 0.18 ± 0.14 μg/mL. The volume of distribution and clearance values were quite low, but reasonably homogenous among individuals (Vdss 142.31 ± 55.08 mL/kg and ClB 4.38 ± 0.95 mL/kg/h, respectively). The IV administration of meloxicam in buffalo calves shows encouraging effects represented by significant and prolonged analgesic effects, significant reduction of SCL as well as similar pharmacokinetic profile to bovine calves.