An integrated map of HIV-human protein complexes that facilitate viral infection

Recent proteomic and genetic studies have aimed to identify a complete network of interactions between HIV and human proteins and genes. This HIV-human interaction network provides invaluable information as to how HIV exploits the host machinery and can be used as a starting point for further functi...

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Bibliographic Details
Published in:	PloS one Vol. 9; no. 5; p. e96687
Main Authors:	Emig-Agius, Dorothea, Olivieri, Kevin, Pache, Lars, Shih, Hsin Ling, Pustovalova, Olga, Bessarabova, Marina, Young, John A T, Chanda, Sumit K, Ideker, Trey
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 09-05-2014 Public Library of Science (PLoS)
Subjects:	Analysis Bioengineering Bioinformatics Biology and life sciences Cytoskeleton Deoxyribonucleic acid DNA DNA biosynthesis DNA repair DNA replication Drug resistance Gene regulation Genes Genomes Health aspects HEK293 Cells HIV HIV infections HIV Infections - genetics HIV Infections - metabolism HIV Infections - virology HIV-1 - genetics HIV-1 - metabolism HIV-1 - physiology Host-Pathogen Interactions Human immunodeficiency virus Human Immunodeficiency Virus Proteins - genetics Human Immunodeficiency Virus Proteins - metabolism Humans Immunology Infection Infections Jurkat Cells Kinases Medical research Medicine and Health Sciences Ontology Propagation Protein Binding Protein Interaction Mapping - methods Protein Interaction Maps Proteins Proteomics Proteomics - methods RNA Interference RNA-mediated interference Screens Signal Transduction Studies Transcription Viral infections Los Angeles California La Jolla California California United States > US
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Summary:	Recent proteomic and genetic studies have aimed to identify a complete network of interactions between HIV and human proteins and genes. This HIV-human interaction network provides invaluable information as to how HIV exploits the host machinery and can be used as a starting point for further functional analyses. We integrated this network with complementary datasets of protein function and interaction to nominate human protein complexes with likely roles in viral infection. Based on our approach we identified a global map of 40 HIV-human protein complexes with putative roles in HIV infection, some of which are involved in DNA replication and repair, transcription, translation, and cytoskeletal regulation. Targeted RNAi screens were used to validate several proteins and complexes for functional impact on viral infection. Thus, our HIV-human protein complex map provides a significant resource of potential HIV-host interactions for further study.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Current address: Department of Microbiology, Immunology & Molecular Genetics, Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California at Los Angeles, Los Angeles, California, United States of America Conceived and designed the experiments: DEA TI. Performed the experiments: DEA KO HLS. Analyzed the data: DEA LP KO OP. Contributed reagents/materials/analysis tools: MB. Wrote the paper: DEA TI KO SC JY. Competing Interests: Dorothea Emig-Agius, Olga Pustovalova, and Marina Bessarabova are employees of Thomson Reuters. No funding was provided by Thomson Reuters and the company had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0096687