Micronized Copper Wood Preservatives: Efficacy of Ion, Nano, and Bulk Copper against the Brown Rot Fungus Rhodonia placenta

• Published in: PloS one Vol. 10; no. 11; p. e0142578
• Main Authors: Civardi, Chiara, Schubert, Mark, Fey, Angelika, Wick, Peter, Schwarze, Francis W M R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10-11-2015; Public Library of Science (PLoS)
• Subjects: Acid production; Ammonium; Antifungal activity; Antifungal agents; Azoles; Biocides; Biodegradation; Brown rot; Carbonates; Cell walls; Copper; Copper azole; Coriolaceae; Effectiveness; Enzymes; Formulations; Fungi; Fungicides; Gene expression; Heterocyclic compounds; Laccase; Membranes; Mycoses - microbiology; Mycoses - prevention & control; Nanoparticles; Oxalic acid; Plant Diseases - microbiology; Plant Diseases - prevention & control; Preservation, Biological; Preservatives; Rhodonia placenta; Studies; Wood; Wood - microbiology; Wood preservatives; Wood-decaying fungi; Switzerland
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0142578

Recently introduced micronized copper (MC) formulations, consisting of a nanosized fraction of basic copper (Cu) carbonate (CuCO3·Cu(OH)2) nanoparticles (NPs), were introduced to the market for wood protection. Cu NPs may presumably be more effective against wood-destroying fungi than bulk or ionic Cu compounds. In particular, Cu- tolerant wood-destroying fungi may not recognize NPs, which may penetrate into fungal cell walls and membranes and exert their impact. The objective of this study was to assess if MC wood preservative formulations have a superior efficacy against Cu-tolerant wood-destroying fungi due to nano effects than conventional Cu biocides. After screening a range of wood-destroying fungi for their resistance to Cu, we investigated fungal growth of the Cu-tolerant fungus Rhodonia placenta in solid and liquid media and on wood treated with MC azole (MCA). In liquid cultures we evaluated the fungal response to ion, nano and bulk Cu distinguishing the ionic and particle effects by means of the Cu2+ chelator ammonium tetrathiomolybdate (TTM) and measuring fungal biomass, oxalic acid production and laccase activity of R. placenta. Our results do not support the presence of particular nano effects of MCA against R. placenta that would account for an increased antifungal efficacy, but provide evidence that attribute the main effectiveness of MCA to azoles.