Comparative characterization of bacterial communities in geese consuming of different proportions of ryegrass

• Published in: PloS one Vol. 14; no. 10; p. e0223445
• Main Authors: Guo, Baodi, Li, Dianhui, Zhou, Beibei, Jiang, Yong, Bai, Hao, Zhang, Yang, Xu, Qi, Zhao, Wenming, Chen, Guohong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25-10-2019; Public Library of Science (PLoS)
• Subjects: Abdominal Fat; Amino acids; Animal Feed; Animals; Aquatic birds; Bacteria - classification; Bacteroidetes; Biodiversity; Biology and Life Sciences; Body weight; Carbohydrate metabolism; Carbohydrates; Cellulose; Communities; Deoxyribonucleic acid; Diet; Dietary supplements; DNA; Enzymes; Feces; Firmicutes; Gastrointestinal Microbiome; Geese; Geese - microbiology; Geese - physiology; Grasses; Health aspects; Intestinal microflora; Intestine; Intestines; Laboratories; Lipid metabolism; Lipids; Lolium; Male; Meat; Medicine and Health Sciences; Metabolic pathways; Metabolism; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Next-generation sequencing; Obesity; Plants; Polysaccharides; Relative abundance; Ruminococcaceae; Technology; Vertebrates; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0223445

Geese are extremely well-adapted to utilizing plant-derived roughage in their diet, so the grass must be added to commercial diets under intensive rearing systems. However, it is unclear whether the gut microbiota will change significantly when adding different proportions of ryegrass. In this study, 240 healthy male Yangzhou geese (28 days old) with similar body weights were randomly divided into four groups and fed different proportions grass (CK, whole commercial diets; EG1, ryegrass: commercial diets = 1.5:1; EG2, ryegrass: commercial diets = 2:1; EG3, ryegrass: commercial diets = 3:1) respectively. When the geese grew to 70 days old, their intestines were collected and high-throughput sequencing technology was performed to investigate the microbial diversity in the caecum of geese with different dietary supplements. There was no obvious change in the alpha diversity of gut microbiota of geese with ryegrass intake (P > 0.05) and the composition of dominant bacterium (including Bacteroidetes and Firmicutes) was also similar. However, the ratio between Firmicutes and Bacteroidetes was remarkably reduced with ryegrass intake (P < 0.05), and the relative abundance of 30 operational taxonomic units (OTUs) significantly differed. Additionally, the content of cellulose-degrading microbiota such as Ruminiclostridium and Ruminococcaceae UCG-010 were significantly increased in geese fed with increasing amounts of grass. Finally, the functional profiles of the goose gut microbiota were explored using the PICRUSt tool. Carbohydrate metabolism and amino acid metabolism were dominant metabolic pathways. Lipid metabolism was significantly increased in EG3 compared that in the CK group (P < 0.05). Interestingly, Turicibacter and Parasutterella may have affected abdominal fat deposition as grass intake increased. Taken together, although the diversity of bacterial communities was similar in geese fed with different proportions of ryegrass, cellulose-degrading microbiota (Ruminiclostridium and Ruminococcaceae UCG-010) were abundant and the lipid metabolic pathway was enriched, which may reduce abdominal fat accumulation in high-ryegrass fed geese.