MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1

• Published in: PloS one Vol. 8; no. 5; p. e63142
• Main Authors: Zhou, Chang, Liu, Guobing, Wang, Lijing, Lu, Yanxia, Yuan, Li, Zheng, Lin, Chen, Fang, Peng, Fanli, Li, Xuenong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16-05-2013; Public Library of Science (PLoS)
• Subjects: Animals; Antineoplastic Agents - therapeutic use; Apoptosis; Biology; Bladder cancer; Blotting, Western; Breast cancer; Cancer; Cancer metastasis; Cell Cycle - drug effects; Cell Cycle - genetics; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell growth; Cell Line, Tumor; Cell migration; Cell Proliferation - drug effects; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - therapy; Gastroenterology; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - genetics; Growth; Humans; In Vitro Techniques; Inhibition; Invasiveness; Laboratories; Liver; Luciferase; Lung cancer; Lymphatic system; Medical prognosis; Medical research; Medicine; Membrane Proteins - genetics; Membrane Proteins - metabolism; Metastases; Metastasis; Mice; MicroRNA; MicroRNAs; MicroRNAs - antagonists & inhibitors; MicroRNAs - genetics; miRNA; mRNA; Pathology; Phosphatase; Protein Tyrosine Phosphatases - genetics; Protein Tyrosine Phosphatases - metabolism; Proteins; Target recognition; Tissues; Tumor suppressor genes; Xenograft Model Antitumor Assays; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0063142

MicroRNAs (miRNAs) have been suggested to play a vital role in regulate tumor progression and invasion. However, the expression of miR-339-5p in colorectal cancer and its effects are not known. Here, we report that miR-339-5p is a tumor suppressor by regulating expression of PRL-1. In this study, we showed that downregulated miR-339-5p levels in colorectal cancer tissues and highly invasive CRC cell lines. Furthermore, enhancing the expression of miR-339-5p inhibited CRC cell growth, migration and invasion in vitro and suppressed tumor growth in vivo. We then screened and identified a novel miR-339-5p target, phosphatases of regenerating liver-1 1 (PRL-1), and it was further confirmed by luciferase assay. Overexpression of miR-339-5p would also reduce the expression of PRL-1 mRNA and protein. The reduced PRL-1 expression was associated with low expression of phosphorylated-extracellular signal-regulated kinase1/2 (p-ERK1/2). Conversely, reduction of miR-339-5p by inhibitors in cells stimulated these phenotypes. In conclusion, our results demonstrate that miR-339-5p functions as a tumor suppressor and plays a role in inhibiting growth and metastasis of CRC cells through targeting PRL-1 and regulating p-ERK1/2 .These findings suggest that miR-339-5p may be useful as a new potential therapeutic target for CRC.