In vitro cytotoxic and antimicrobial activities of Erythrina suberosa (Roxb) bark

• Published in: Journal of pharmacy & bioallied science Vol. 12; no. 2; pp. 210 - 216
• Main Authors: Ahmed, Zubair, Aziz, Sohail, Alauddin, Syed, Mohiuddin, Syed, Javed, Adil, Ahmed, Rizwan, Bitar, Ahmad, Sheikh Ghadzi, Siti
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01-04-2020; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: Acetic acid; Antifungal activity; Antifungal agents; antimicrobial; Antimicrobial activity; Antimicrobial agents; Apoptosis; Bark; Cancer; Candida krusei; Cefixime; Clotrimazole; Cytotoxicity; Dichloromethane; Drug dosages; Drug resistance; Erythrina; erythrina suberosa (roxb.); Esters; Ethyl acetate; extraction; Flavonoids; Flowers & plants; Lethality; Leukemia; Methanol; Methicillin; Original; pharmacological screening; Plant extracts; Saponins; Staphylococcus aureus; Strains (organisms); Studies; toxicity study; Vincristine; Pakistan; Antimicrobial; toxicity study; pharmacological screening; extraction; Erythrina suberosa (Roxb.)
• ISSN: 0975-7406; 0976-4879; 0975-7406
• DOI: 10.4103/jpbs.jpbs_223_19

Background: The study was focused on evaluating cytotoxic and antimicrobial activities of Erythrina suberosa (Roxb.) bark through in vitro pharmacological screening. Materials and Methods: The bark was extracted using different solvents, for example, dichloromethane, ethyl acetate, methanol, and aqueous for obtaining the organic fractions. These organic fractions were then evaluated for their cytotoxic and antimicrobial activity compared with the standard. Cefixime was used as the standard for antibacterial assay, whereas clotrimazole was used as the standard for antifungal activities. Bacterial strains used were Staphylococcus aureus and methicillin-resistant S. aureus (MRSA), whereas for antifungal activities Candida albicans, Candida parapsilosis, and Candida krusei strains were used. Results: The organic fractions obtained were evaluated for their cytotoxic and antimicrobial activities. In cytotoxic assay (Brine shrimp lethality assay), dichloromethane fraction was the most potent with LD50 of 47.63, whereas aqueous, methanol, and ethyl acetate fractions showed LD50 of 121.74, 422.2, and 201.96, respectively. Similarly, for antibacterial assay, dichloromethane fraction showed 32.2mm zone of inhibition against MRSA in comparison with standard cefixime (zone of inhibition, 30.5mm). A minimal zone of inhibition with crude saponins (13.1 and 12.2mm) was observed against C. albicans in comparison to standard (cefixime) with a zone of inhibition of 28.5mm. No prominent results were observed against C. parapsilosis and C. krusei strains. Conclusion: The study was based on the plant from Indo-Pak origin, and it has shown some prominent cytotoxic and antibacterial activities. Although the results of this study have provided a basic idea about the efficacy of plant extract, still more explanatory and high-scale studies can be beneficial for elaborating the cytotoxic and antimicrobial activities of this plant.