Pep-1 Peptide-Modified Liposomal Carriers for Intracellular Delivery of Gold Nanoparticles

• Published in: Chemical & Pharmaceutical Bulletin Vol. 59; no. 1; pp. 109 - 112
• Main Authors: Kang, Myung Joo, Lee, Sangyeop, Kim, Bo Kyun, Eum, Jae Yoon, Park, Sang Han, Kang, Min Hyung, Oh, Chil Hwan, Choo, Jaebum, Choi, Young Wook
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01-01-2011; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Cell Line, Tumor; Cysteamine - analogs & derivatives; Cysteamine - chemistry; Ethanol - chemistry; Gold - chemistry; gold nanoparticle; Humans; intracellular delivery; liposome; Liposomes - chemistry; Metal Nanoparticles - administration & dosage; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; nanocarrier; Particle Size; Pep-1 peptide; Peptides - chemistry; Surface Plasmon Resonance
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.59.109

A Pep-1 peptide-modified liposomal (Pep1-Lipo) carrier system was investigated to increase the intracellular delivery of gold nanoparticles (Au NPs). Au NPs with a mean diameter of 13 nm were successfully encapsulated into the inner aqueous compartment of the novel carrier using an ethanol injection technique, reserving the distinctive optical characteristics of the surface plasmon resonance peak around 530 nm. The Au NP-loaded liposomal carrier was physically characterized as 150—170 nm in size and 45 mV in zeta potential. Dark field microscopic observation demonstrated that in vitro cellular association and/or translocation of the nanoprobes into the cells was increased by Pep1-Lipo carriers compared to bare Au NPs. In conclusion, this novel liposomal formulation is a promising platform for the intracellular delivery of metallic nanoprobes including Au NPs.