Amelioration of Hyperbilirubinemia in Gunn Rats after Transplantation of Human Induced Pluripotent Stem Cell-Derived Hepatocytes

• Published in: Stem cell reports Vol. 5; no. 1; pp. 22 - 30
• Main Authors: Chen, Yong, Li, Yanfeng, Wang, Xia, Zhang, Wei, Sauer, Vanessa, Chang, Chan-Jung, Han, Bing, Tchaikovskaya, Tatyana, Avsar, Yesim, Tafaleng, Edgar, Madhusudana Girija, Sanal, Tar, Krisztina, Polgar, Zsuzsanna, Strom, Stephen, Bouhassira, Eric E., Guha, Chandan, Fox, Ira J., Roy-Chowdhury, Jayanta, Roy-Chowdhury, Namita
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14-07-2015; Elsevier
• Subjects: Animals; Bilirubin - blood; Cell- and Tissue-Based Therapy; Crigler-Najjar Syndrome - blood; Crigler-Najjar Syndrome - pathology; Crigler-Najjar Syndrome - therapy; Glucuronosyltransferase - deficiency; Glucuronosyltransferase - genetics; Hepatocytes - transplantation; Humans; Hyperbilirubinemia - blood; Hyperbilirubinemia - genetics; Hyperbilirubinemia - therapy; Induced Pluripotent Stem Cells - transplantation; Liver - pathology; Liver - surgery; Medicin och hälsovetenskap; Rats; Rats, Gunn
• ISSN: 2213-6711; 2213-6711
• DOI: 10.1016/j.stemcr.2015.04.017

Hepatocyte transplantation has the potential to cure inherited liver diseases, but its application is impeded by a scarcity of donor livers. Therefore, we explored whether transplantation of hepatocyte-like cells (iHeps) differentiated from human induced pluripotent stem cells (iPSCs) could ameliorate inherited liver diseases. iPSCs reprogrammed from human skin fibroblasts were differentiated to iHeps, which were transplanted into livers of uridinediphosphoglucuronate glucuronosyltransferase-1 (UGT1A1)-deficient Gunn rats, a model of Crigler-Najjar syndrome 1 (CN1), where elevated unconjugated bilirubin causes brain injury and death. To promote iHep proliferation, 30% of the recipient liver was X-irradiated before transplantation, and hepatocyte growth factor was expressed. After transplantation, UGT1A1+ iHep clusters constituted 2.5%–7.5% of the preconditioned liver lobe. A decline of serum bilirubin by 30%–60% and biliary excretion of bilirubin glucuronides indicated that transplanted iHeps expressed UGT1A1 activity, a postnatal function of hepatocytes. Therefore, iHeps warrant further exploration as a renewable source of hepatocytes for treating inherited liver diseases.
•Human skin fibroblast-derived iPSCs were differentiated to hepatocyte-like iHeps•iHeps were transplanted into Gunn rats, a model of Crigler-Najjar syndrome 1•Engraftment of the iHeps in Gunn rat livers reduced serum bilirubin levels•iHeps may be potentially useful in treating liver-based metabolic disorders
Roy-Chowdhury and colleagues show that human iPSCs differentiated to hepatocyte-like iHep cells engraft into the liver of UGT1A1-deficient Gunn rats, a model of Crigler-Najjar syndrome 1, where hyperbilirubinemia causes brain injury and death. After iHep transplantation, serum bilirubin declined by 30%–60% and bilirubin glucuronides appeared in bile, indicating that the engrafted iHeps expressed UGT1A1, a postnatal hepatocyte function.