Protective effects of 5-aminolevulinic acid on heat stress in bovine mammary epithelial cells

• Published in: Animal bioscience Vol. 34; no. 6; pp. 1006 - 1013
• Main Authors: Islam, Md Aminul, Noguchi, Yoko, Taniguchi, Shin, Yonekura, Shinichi
• Format: Journal Article
• Language: English
• Published: Asian - Australasian Association of Animal Production Societies 01-06-2021; Animal Bioscience; Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: 5-aminolevulinic acid; Analysis; Antioxidants; Apoptosis; bovine mammary epithelial cells; Cattle; Diseases; endoplasmic reticulum stress; Epithelial cells; Ethylenediaminetetraacetic acid; Genes; heat stress; Heat stress disorders; Heme; Mammary glands; Milk; Milk production; oxidative stress; Physiological aspects; Prevention; RNA; 축산학; Japan
• ISSN: 2765-0189; 2765-0235; 2765-0235
• DOI: 10.5713/ajas.20.0349

Objective: Cells have increased susceptibility to activation of apoptosis when suffering heat stress (HS). An effective supplementation strategy to mimic heat-induced apoptosis of bovine mammary epithelial cells (MECs) is necessary to maintain optimal milk production. This study aimed to investigate possible protective effects of the anti-apoptotic activity of 5-aminolevulinic acid (5-ALA) against HS-induced damage of bovine MECs.Methods: Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 μM for 24 h followed by HS (42.5°C for 24 h and 48 h). Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress genes.Results: We found that 5-ALA induces cytoprotection via inhibition of apoptosis markers after HS-induced damage. Pretreatment of bovine MECs with 5-ALA resulted in dramatic upregulation of mRNA for nuclear factor erythroid-derived 2-like factor 2, heme oxygenase-1, and NAD(P)H quinone oxidoreductase 1, all of which are antioxidant stress genes. Moreover, 5-ALA pretreatment significantly suppressed HS-induced ER stress-associated markers, glucose-regulated protein 78, and C/EBP homologous protein expression levels.Conclusion: 5-ALA can ameliorate the ER stress in heat stressed bovine MEC via enhancing the expression of antioxidant gene.