Enhanced triacylglycerol catabolism by carboxylesterase 1 promotes aggressive colorectal carcinoma

• Published in: The Journal of clinical investigation Vol. 131; no. 11; pp. 1 - 16
• Main Authors: Capece, Daria, D'Andrea, Daniel, Begalli, Federica, Goracci, Laura, Tornatore, Laura, Alexander, James L, Di Veroli, Alessandra, Leow, Shi-Chi, Vaiyapuri, Thamil S, Ellis, James K, Verzella, Daniela, Bennett, Jason, Savino, Luca, Ma, Yue, McKenzie, James S, Doria, Maria Luisa, Mason, Sam E, Chng, Kern Rei, Keun, Hector C, Frost, Gary, Tergaonkar, Vinay, Broniowska, Katarzyna, Stunkel, Walter, Takats, Zoltan, Kinross, James M, Cruciani, Gabriele, Franzoso, Guido
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 01-06-2021
• Subjects: Adaptation; Animal models; Biomedical research; Body weight; Cancer; Carboxylesterase; Carboxylic Ester Hydrolases - genetics; Carboxylic Ester Hydrolases - metabolism; Cell survival; Clinical outcomes; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Development and progression; Energy metabolism; Epithelial cells; Esterases; Fat metabolism; Fatty acids; Female; Gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Genetic aspects; Glucose; Health aspects; Hepatocyte nuclear factor 4; Humans; Inflammation; Life Sciences; Lipids; Lipolysis; Male; Medical prognosis; Metabolism; Metabolites; Metastasis; Microenvironments; Mutation; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; NF-κB protein; Obesity; Overweight; Oxidation; Physiological aspects; Triglycerides; Triglycerides - genetics; Triglycerides - metabolism; Tumors; United States; Oncology; NF-kappaB; Metabolism; Colorectal cancer
• ISSN: 1558-8238; 0021-9738; 1558-8238
• DOI: 10.1172/JCI137845

The ability to adapt to low-nutrient microenvironments is essential for tumor cell survival and progression in solid cancers, such as colorectal carcinoma (CRC). Signaling by the NF-κB transcription factor pathway associates with advanced disease stages and shorter survival in patients with CRC. NF-κB has been shown to drive tumor-promoting inflammation, cancer cell survival, and intestinal epithelial cell (IEC) dedifferentiation in mouse models of CRC. However, whether NF-κB affects the metabolic adaptations that fuel aggressive disease in patients with CRC is unknown. Here, we identified carboxylesterase 1 (CES1) as an essential NF-κB-regulated lipase linking obesity-associated inflammation with fat metabolism and adaptation to energy stress in aggressive CRC. CES1 promoted CRC cell survival via cell-autonomous mechanisms that fuel fatty acid oxidation (FAO) and prevent the toxic build-up of triacylglycerols. We found that elevated CES1 expression correlated with worse outcomes in overweight patients with CRC. Accordingly, NF-κB drove CES1 expression in CRC consensus molecular subtype 4 (CMS4), which is associated with obesity, stemness, and inflammation. CES1 was also upregulated by gene amplifications of its transcriptional regulator HNF4A in CMS2 tumors, reinforcing its clinical relevance as a driver of CRC. This subtype-based distribution and unfavorable prognostic correlation distinguished CES1 from other intracellular triacylglycerol lipases and suggest CES1 could provide a route to treat aggressive CRC.