Development of a Bioluminescent Bacteria Sheet for the Measurement of Oxygen Concentration

The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm. Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it. The r...

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Bibliographic Details
Published in:	Analytical Sciences Vol. 29; no. 2; pp. 267 - 269
Main Authors:	SASAKI, Satoshi, MOCHIZUKI, Mika, IGARASHI, Makoto
Format:	Journal Article
Language:	English
Published:	Singapore The Japan Society for Analytical Chemistry 2013 Springer Nature Singapore Japan Science and Technology Agency
Subjects:	Alginates - chemistry Analytical Chemistry Biofilms Biofilms - growth & development Bioluminescence Biosensing Techniques - methods Chemistry Dissolved oxygen Fabrication Glucuronic Acid - chemistry Hexuronic Acids - chemistry Oxygen Oxygen - metabolism Photobacterium - cytology Photobacterium - metabolism Pseudomonas aeruginosa - physiology Thin films
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Summary:	The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm. Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it. The relationship between the dissolved oxygen (DO) concentration in the biofilm and luminescence from the sheet was examined. As a conclusion, the alginate-gel film method was effective in fabricating a DO-monitoring thin film.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0910-6340 1348-2246
DOI:	10.2116/analsci.29.267