Establishment of a Confluent Cardiomyocyte Culture in a Cylindrical Microchannel

We established a confluent cardiomyocyte culture method using an 800-μm diameter cylindrical microchannel in this report. This was realized by introducing cardiomyocytes 2 times before and after turning over a microchip. The optimum condition was starting the flowing medium 2.0 h after seeding and f...

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Bibliographic Details
Published in:	Analytical Sciences Vol. 27; no. 9; p. 957
Main Authors:	TANAKA, Yo, AKAIKE, Hiroto, SUGII, Yasuhiko, KITAMORI, Takehiko
Format:	Journal Article
Language:	English
Published:	Singapore The Japan Society for Analytical Chemistry 2011 Springer Nature Singapore Japan Science and Technology Agency
Subjects:	Analytical Chemistry Animals Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cells, Cultured Chemistry Infusion Pumps Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Rats Rats, Wistar
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Summary:	We established a confluent cardiomyocyte culture method using an 800-μm diameter cylindrical microchannel in this report. This was realized by introducing cardiomyocytes 2 times before and after turning over a microchip. The optimum condition was starting the flowing medium 2.0 h after seeding and flowing the medium at 1.0 μL/min. By applying this technology to a cardiomyocyte-based spherical heart pump device, one may develop self-fluid regulated devices that could be applied for implantable or circulation analysis device on a chip.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0910-6340 1348-2246
DOI:	10.2116/analsci.27.957