Detecting the movement and spawning activity of bigheaded carps with environmental DNA

Bigheaded carps are invasive fishes threatening to invade the Great Lakes basin and establish spawning populations, and have been monitored using environmental DNA (eDNA). Not only does eDNA hold potential for detecting the presence of species, but may also allow for quantitative comparisons like re...

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Bibliographic Details
Published in:	Molecular ecology resources Vol. 16; no. 4; pp. 957 - 965
Main Authors:	Erickson, Richard A., Rees, Christopher B., Coulter, Alison A., Merkes, Christopher M., McCalla, Sunnie G., Touzinsky, Katherine F., Walleser, Liza, Goforth, Reuben R., Amberg, Jon J.
Format:	Journal Article
Language:	English
Published:	England Blackwell Publishing Ltd 01-07-2016 Wiley Subscription Services, Inc John Wiley and Sons Inc
Subjects:	Animals Asian carp bigheaded carp Carps - classification Carps - genetics Carps - physiology Deoxyribonucleic acid DNA DNA - genetics DNA - isolation & purification environmental monitoring Environmental Monitoring - methods fisheries management Indiana invasive species detection Locomotion Metagenomics - methods Reproduction Resource RESOURCE ARTICLES Rivers Sequence Analysis, DNA Water - chemistry Asian carp bigheaded carp environmental monitoring fisheries management invasive species detection
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Summary:	Bigheaded carps are invasive fishes threatening to invade the Great Lakes basin and establish spawning populations, and have been monitored using environmental DNA (eDNA). Not only does eDNA hold potential for detecting the presence of species, but may also allow for quantitative comparisons like relative abundance of species across time or space. We examined the relationships among bigheaded carp movement, hydrography, spawning and eDNA on the Wabash River, IN, USA. We found positive relationships between eDNA and movement and eDNA and hydrography. We did not find a relationship between eDNA and spawning activity in the form of drifting eggs. Our first finding demonstrates how eDNA may be used to monitor species abundance, whereas our second finding illustrates the need for additional research into eDNA methodologies. Current applications of eDNA are widespread, but the relatively new technology requires further refinement.
Bibliography:	U.S. Environmental Protection Agency - Great Lakes Restoration Initiative ArticleID:MEN12533 Fig. S1 Observed copy numbers along sampling transects. Fig. S2 Observed copy numbers and flow through time at the three study sites. Fig. S3 Flow vs. egg density for all three sites. Fig. S4 Relationship between flow and the count of unique carp passing the Americus study site each day. Fig. S5 Wabash River discharge data for 2013 at the three transect locations (French Post, Americus, and Mascouten) and one major tributary (Tippecanoe's confluence, which is located immediately downriver from Americus). Table S1 Posterior distributions for regression parameters from the multilevel model used to examine the relationship between eDNA copy number and telemetry U.S. Geological Survey istex:249E3DDCB9C7894A7B4478EDAD682CB7E6CEF5D1 ark:/67375/WNG-G1QK8VM7-R Indiana Department of Natural Resources ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. The copyright line for this article was changed on 25th June, 2016 after original online publication These authors also contributed equally to this work.
ISSN:	1755-098X 1755-0998 1755-0998
DOI:	10.1111/1755-0998.12533