A subset of sweet-sensing neurons identified by IR56d are necessary and sufficient for fatty acid taste

• Published in: PLoS genetics Vol. 13; no. 11; p. e1007059
• Main Authors: Tauber, John M, Brown, Elizabeth B, Li, Yuanyuan, Yurgel, Maria E, Masek, Pavel, Keene, Alex C
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09-11-2017; Public Library of Science (PLoS)
• Subjects: Animals; Biology and Life Sciences; Drosophila melanogaster - genetics; Drosophila melanogaster - physiology; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Fatty acids; Fatty Acids, Nonesterified - genetics; Fatty Acids, Nonesterified - metabolism; Gene Expression Regulation; Medicine and Health Sciences; Neurons; Physical Sciences; Physiological aspects; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Ionotropic Glutamate - genetics; Receptors, Ionotropic Glutamate - metabolism; Research and Analysis Methods; Sensory Receptor Cells - metabolism; Social Sciences; Sugars - metabolism; Taste (Sense); Taste - genetics; Taste - physiology; Taste Perception - genetics
• ISSN: 1553-7404; 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1007059

Fat represents a calorically potent food source that yields approximately twice the amount of energy as carbohydrates or proteins per unit of mass. The highly palatable taste of free fatty acids (FAs), one of the building blocks of fat, promotes food consumption, activates reward circuitry, and is thought to contribute to hedonic feeding underlying many metabolism-related disorders. Despite a role in the etiology of metabolic diseases, little is known about how dietary fats are detected by the gustatory system to promote feeding. Previously, we showed that a broad population of sugar-sensing taste neurons expressing Gustatory Receptor 64f (Gr64f) is required for reflexive feeding responses to both FAs and sugars. Here, we report a genetic silencing screen to identify specific populations of taste neurons that mediate fatty acid (FA) taste. We find neurons identified by expression of Ionotropic Receptor 56d (IR56d) are necessary and sufficient for reflexive feeding response to FAs. Functional imaging reveals that IR56d-expressing neurons are responsive to short- and medium-chain FAs. Silencing IR56d neurons selectively abolishes FA taste, and their activation is sufficient to drive feeding responses. Analysis of co-expression with Gr64f identifies two subpopulations of IR56d-expressing neurons. While physiological imaging reveals that both populations are responsive to FAs, IR56d/Gr64f neurons are activated by medium-chain FAs and are sufficient for reflexive feeding response to FAs. Moreover, flies can discriminate between sugar and FAs in an aversive taste memory assay, indicating that FA taste is a unique modality in Drosophila. Taken together, these findings localize FA taste within the Drosophila gustatory center and provide an opportunity to investigate discrimination between different categories of appetitive tastants.