Meningococcal factor H-binding protein vaccines with decreased binding to human complement factor H have enhanced immunogenicity in human factor H transgenic mice

• Published in: Vaccine Vol. 31; no. 46; pp. 5451 - 5457
• Main Authors: Rossi, Raffaella, Granoff, Dan M, Beernink, Peter T
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 04-11-2013; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Amino Acid Substitution; Animal model; Animals; antibacterial properties; Antibodies, Bacterial - blood; antigens; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Applied microbiology; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bactericidal; Bacteriology; Biological and medical sciences; Blood Bactericidal Activity; blood serum; complement; cross reaction; Disease; Female; fHbp; Fundamental and applied biological sciences. Psychology; Human factors; Humans; Immunogenicity; immunoglobulin G; Immunoglobulin G - blood; Life sciences; Male; Meningococcal Vaccines - administration & dosage; Meningococcal Vaccines - genetics; Meningococcal Vaccines - immunology; Mice; Mice, Transgenic; Microbiology; Miscellaneous; Mutant fHbp vaccine; Mutant Proteins - genetics; Mutant Proteins - immunology; Mutants; Mutation; Neisseria meningitidis; Protein Binding; Proteins; Rodents; serotypes; Studies; Sub-family A; Transgenic animals; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Variant group 2; Europe; Sub-family A; Animal model; fH; Bactericidal; Variant group 2; fHbp; Mutant fHbp vaccine; Human; Neisseriaceae; Rodentia; Transgenic animal; Complement; Neisseria meningitidis; Vaccine; Binding protein; Vertebrata; Mammalia; Immunogenicity; Mouse; Bacteria; Micrococcales; Mutation
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2013.08.099

Highlights • We constructed two meningococcal factor H binding protein (fHbp) sub-family B mutants. • Both mutants had decreased binding of human factor H. • The mutations did not impair protective antibody responses in mice. • The mutants elicited significantly augmented antibody responses in human factor H transgenic mice. • Mutants with decreased factor H binding may lead to improved vaccines for humans.