Biological activities of a recombinant fortilin from Fenneropenaeus merguiensis

• Published in: PloS one Vol. 15; no. 10; p. e0239672
• Main Authors: Kedjarune-Leggat, Ureporn, Saetan, Uraipan, Khongsaengkaeo, Anchana, Suwannarat, Sudarat, Deachamag, Panchalika, Wonglapsuwan, Monwadee, Pornprasit, Rawiwan, Thongkamwitoon, Wanwisa, Phumklai, Parujee, Chaichanan, Jirapan, Chotigeat, Wilaiwan
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 01-10-2020; Public Library of Science (PLoS)
• Subjects: Amino acids; Apoptosis; Bioinformatics; Biology and Life Sciences; Biotechnology; Care and treatment; Cell growth; Cell proliferation; Cloning; Composition; Cytokines; Dental materials; Dental pulp; Dentistry; E coli; Fenneropenaeus merguiensis; Fragments; Genomes; Health aspects; Histamine; Homology; Inflammation; Medical materials; Medicine and Health Sciences; Mineralization; Ovarian cancer; Penaeidae; Physical Sciences; Proteins; Recombinant proteins; Research and analysis methods; Teeth; Tooth diseases; Toxicity; Tumor necrosis factor-α; Tumor proteins; Thailand
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0239672

Human Fortilin, an antiapoptotic protein, has also been implicated in several diseases; however, several potential uses of fortilin have also been proposed. Bearing the implications of fortilin in mind, fortilin analog, which has no complication with diseases, is required. Since a recombinant full-length fortilin from Fenneropenaeus merguiensis (rFm-Fortilin (FL)) reported only 44% (3e.sup.-27) homologous to human fortilin, therefore the biological activities of the Fm-Fortilin (FL) and its fragments (F2, F12, and F23) were investigated for potential use against HEMA toxicity from filling cement to pulp cell. The rFm-Fortilin FL, F2, 12, and F23 were expressed and assayed for proliferation activity. The rFm-Fortilin (FL) showed proliferation activity on human dental pulp cells (HDPCs) and protected the cells from 2-hydroxy-ethyl methacrylate (HEMA) at 1-20 ng/ml. In contrast, none of the rFm-Fortilin fragments promoted HDPC growth that may be due to a lack of three conserved amino acid residues together for binding with the surface of Rab GTPase for proliferative activity. In addition, rFm-Fortilin (FL) activated mineralization and trend to suppressed production of proinflammatory cytokines, including histamine (at 10 ng/ml) and TNF-[alpha] (at 100 ng/ml). Besides, the rFm-Fortilin (FL) did not mutate the Chinese hamster ovary (CHO) cell. Therefore, the rFm-Fortilin (FL) has the potential use as a supplementary medical material to promote cell proliferation in patients suffering severe tooth decay and other conditions.