Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer

• Published in: PloS one Vol. 16; no. 7; p. e0254921
• Main Authors: Pichu, Sivakamasundari, Vimalraj, Selvaraj, Viswanathan, Vijay
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 22-07-2021; Public Library of Science (PLoS)
• Subjects: Analysis; Angiogenesis; Apoptosis; Biology and life sciences; Biomarkers; Blood & organ donations; Care and treatment; Caspase-3; Cell growth; Chronic illnesses; Diabetes; Diabetes mellitus; Diabetic foot; Feet; Foot diseases; Gene expression; Genetic aspects; Genomes; Glucose; Hospitals; Hypoxia; Hypoxia-inducible factor 1a; Inflammation; Interleukin 6; Ischemia; Leg ulcers; Medicine and Health Sciences; MicroRNA; MicroRNAs; miRNA; Physiology; Polymerase chain reaction; Research and Analysis Methods; Ribonucleic acid; RNA; Tissues; Tumor necrosis factor-α; Vascular endothelial growth factor; Wound healing; Wounds and injuries; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0254921

Diabetic foot ulcer (DFU) is a major concern in diabetes and its control requires in-depth molecular investigation. The present study aimed to screen the expression of microRNA-210 (miR-210) and its association in hypoxic pathway in DFU patients. The study consists of 3 groups of circulation samples (50 in each group of: healthy volunteers, T2DM and T2DM with DFU) and 2 groups of tissue samples (10 in each group of: control and T2DM with DFU). Expression of miR-210 and hypoxia inducible factor-1 alpha (HIF-1[alpha]), and its responsive genes such as VEGF, TNF-[alpha], IL-6, BCl2, Bax and Caspase 3 were analyzed by RT-PCR, Western blot and ELISA analyses. The HIF-1[alpha] expression decreased in DFU patients with increased miR-210 expression in both circulation and tissue biopsies. The circulatory IL-6 and inflammatory gene TNF-[alpha] expression was increased in DFU compared to healthy controls and T2DM subjects. Further, we found there was no alteration in the angiogenic marker, VEGF expression. In comparison, anti-apoptotic BCl2 was decreased and Bax and Caspase 3 was increased in DFU tissues relative to control. The study showed that there was an inverse relationship between miR-210 and HIF-1[alpha] expression in patients with DFU, indicating that miR-210 may regulate the expression of the hypoxic gene.