Concerning Increase in Antimicrobial Resistance in Shiga Toxin-Producing Escherichia coli Isolated from Young Animals during 1980–2016

• Published in: Microbes and Environments Vol. 32; no. 3; pp. 252 - 259
• Main Authors: Chirila, Flore, Tabaran, Alexandra, Fit, Nicodim, Nadas, George, Mihaiu, Marian, Tabaran, Flaviu, Cătoi, Cornel, Reget, Oana Lucia, Dan, Sorin Daniel
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2017; Japan Science and Technology Agency; the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)
• Subjects: Ampicillin; Animals; antibiotic resistance; Antigens; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; Cassettes; Drug resistance; Drug Resistance, Multiple, Bacterial; E coli; Genes; Genes, Bacterial; health; Integrons; Livestock; Livestock - microbiology; Microbial Sensitivity Tests; Multidrug resistance; Nucleotide sequence; Nucleotides; Phenotypes; Sequencing; Shiga toxin; Shiga toxin-producing E. coli; Shiga-Toxigenic Escherichia coli - drug effects; Shiga-Toxigenic Escherichia coli - isolation & purification; Streptomycin; Sulfonamides; health; antibiotic resistance; E. coli; Shiga toxin-producing E. coli
• ISSN: 1342-6311; 1347-4405
• DOI: 10.1264/jsme2.ME17023

This study was conducted in order to assess the antimicrobial resistance patterns of E. coli isolated from young animals affected between 1980 and 2016. The selected isolates for this study (n=175) carried stx1/stx2 genes and the most prevalent type of pathogenic E. coli found belonged to serogroup O101, antigen (K99)–F41 positive. All STEC-positive isolates were tested for susceptibility to 11 antimicrobials. Multidrug resistance (MDR) increased from 11% during the 1980s to 40% between 2000 and 2016. Resistance to tetracycline and streptomycin was the most frequent co-resistance phenotype (37%). Co-resistance to tetracycline and sulfonamide was found in 21% of E. coli isolates, while the MDR pattern to tetracycline, sulfonamide, and streptomycin was observed in 12% of the strains tested. Only 8% of isolates were co-resistant to tetracycline, ampicillin, streptomycin, and sulfonamide. The most common resistance genes found were those encoding for tetracycline, sulphonamides, and streptomycin, with 54% (n=95) of the tested isolates containing at least one of the genes encoding tetracycline resistance. A total of 87% of E. coli that tested positive for tetracycline (tetA, tetB, and tetC) and sulphonamide (sul1) resistance genes were isolated between 2000 and 2016. A large number of isolates (n=21) carried int1 and a nucleotide sequence analysis revealed that all class 1 integron gene cassettes carried sul1, tet, and dfrA1 resistance genes. An increase was observed in the level of resistance to antimicrobials in Romania, highlighting the urgent need for a surveillance and prevention system for antimicrobial resistance in livestock in Eastern Europe.