Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure

• Published in: Journal of immunology research Vol. 2016; no. 2016; pp. 1 - 9
• Main Authors: Machado, Juliana Reis, Rocha, Laura Penna, Helmo, Fernanda Rodrigues, Corrêa, Rosana Rosa Miranda, Rocha, Lenaldo Branco, Camara, Niels Olsen Saraiva, Teixeira, Vicente de Paula Antunes, da Glória, Maria Aparecida, Rodrigues, Aldo Rogelis Aquiles, da Silva, Aline Cristina Souza, Guimarães, Camila Souza de Oliveira, Pereira Júnior, Carlos Donizete, dos Reis, Marlene Antônia
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2016; Hindawi Limited
• Subjects: Acids; Animals; Biomarkers - metabolism; Chemokine CCL2 - metabolism; Chronic illnesses; Diabetes; Diabetes Mellitus, Experimental - pathology; Diabetes, Gestational - pathology; Female; Females; Fetal Development - physiology; Folic Acid - pharmacology; Gene expression; Hyperglycemia; Hypertension; Hypoxia; Immunology; Interleukin-1 - metabolism; Kidney - immunology; Kidney - pathology; Kinases; Lymphotoxin-alpha - metabolism; Male; Metabolism; Nitric Oxide Synthase Type III - metabolism; Pathology; Physiology; Pregnancy; Rats; Rats, Wistar; Renal Insufficiency - immunology; Renal Insufficiency - pathology; Rodents; Studies; Tumor Necrosis Factor-alpha - metabolism; Vascular Endothelial Growth Factor A - metabolism; Vitamin B
• ISSN: 2314-8861; 2314-7156
• DOI: 10.1155/2016/9151607

Objective. To evaluate the expression of inflammatory markers in experimental renal failure after fetal programming. Methods. The offspring aged two and five months were divided into four groups: CC (control dams, control offspring); DC (diabetic dams, control offspring); CFA (control dams, folic acid offspring, 250 mg/Kg); and DFA (diabetic dams, folic acid offspring). Gene expression of inflammatory markers MCP-1, IL-1, NOS3, TGF-β, TNF-α, and VEGF was evaluated by RT-PCR. Results. MCP-1 was increased in the CFA and DFA groups at two and five months of age, as well as in DC5 when compared to CC5. There was a higher expression of IL-1 in the CFA2, DFA2, and DC2 groups. There was a decrease in NOS3 and an increase in TNF-α in DFA5 in relation to CFA5. The gene expression of TGF-β increased in cases that had received folic acid at two and five months, and VEGF decreased in the CFA5 and DFA5 groups. DC5 showed increased VEGF expression in comparison with CC5. Conclusions. Gestational diabetes mellitus and folic acid both change the expression of inflammatory markers, thus demonstrating that the exposure to harmful agents in adulthood has a more severe impact in cases which underwent fetal reprogramming.