Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome

• Published in: Journal of Bacteriology Vol. 174; no. 10; pp. 3220 - 3226
• Main Authors: Calcutt, M.J. (University of Missouri-Columbia, Columbia, MO), Schmidt, F.J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-05-1992
• Subjects: Amino Acid Sequence; Animals; BACTERIA; Bacterial Proteins - genetics; Bacteriology; Base Sequence; Biological and medical sciences; CHROMOSOME; Chromosome Mapping; Cloning, Molecular; Consensus Sequence; Cosmids; CROMOSOMAS; Deoxyribonucleic acid; DNA; DNA Polymerase III - genetics; DNA Replication - genetics; DNA-Binding Proteins - genetics; Dogs; Endoribonucleases - genetics; FLORA DEL SUELO; FLORE DU SOL; Fundamental and applied biological sciences. Psychology; Genes, Bacterial - genetics; Genetics; Microbiology; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; Phylogeny; Regulatory Sequences, Nucleic Acid; Ribonuclease P; Ribosomal Proteins - genetics; RNA, Catalytic - genetics; Sequence Homology, Nucleic Acid; STREPTOMYCES; Streptomyces - genetics; Streptomyces coelicolor; Actinomycetales; Origin; Streptomycetaceae; Gene; Nucleotide sequence; Streptomyces coelicolor; Sequence alignment; Bacteria; Replication; Actinomycetes; Aminoacid sequence
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.174.10.3220-3226.1992

A 23-kb fragment of the Streptomyces coelicolor chromosome spanning the dnaA region has been isolated as a cosmid clone. Nucleotide sequence analysis of a 5-kb portion shows that the genes for the RNase P protein (rnpA), ribosomal protein L34 (rpmH), the replication initiator protein (dnaA), and the beta subunit of DNA polymerase III (dnaN) are present in the highly conserved gene arrangement found in all eubacterial genomes studied so far. The dnaA--dnaN intergenic region is approximately 1 kb and contains a cluster of at least 12 DnaA boxes with a consensus sequence of TTGTCCACA matching the consensus DnaA box in the phylogenetically related Micrococcus luteus. Two DnaA boxes precede the dnaA sequence. We propose that the chromosomal origin (oriC) of S. coelicolor lies between dnaA and dnaN. In related work, J. Zakrzewska-Czerwinska and H. Schrempf (J. Bacteriol. 174:2688-2693, 1992) have identified the homologous sequence from the closely-related Streptomyces lividans as capable of self-replication