Rapid Detection of Mutation in RRDR of rpo B Gene for Rifampicin Resistance in MDR-Pulmonary Tuberculosis by DNA Sequencing

• Published in: Indian journal of clinical biochemistry Vol. 25; no. 3; pp. 315 - 318
• Main Authors: Patra, Surajeet Kumar, Jain, Anju, Sherwal, B. L., Khanna, Ashwani
• Format: Journal Article
• Language: English
• Published: India Springer-Verlag 01-07-2010; Springer; Springer Nature B.V
• Subjects: Antibiotics; Biochemistry; Biomedical and Life Sciences; Chemistry/Food Science; Codon; Deoxyribonucleic acid; DNA; DNA sequencing; Drug resistance; Genes; Genetic aspects; Genetic research; Life Sciences; Medical research; Medicine, Experimental; Microbiology; Mutation; Mycobacterium; Nucleotide sequencing; Original ; Laboratory Documentation; Original Article; Laboratory Documentation; Pathology; Pulmonary tuberculosis; Rifampin; Tuberculosis; Tuberculosis; rpo B gene; Rifampicin resistance; Multidrug resistance
• ISSN: 0970-1915; 0974-0422
• DOI: 10.1007/s12291-010-0065-3

To detect the site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-TB by DNA sequencing. 50 MDR-TB patients were enrolled in our study after informed written consent. Mycobacterial DNA was extracted from sputum samples by Universal Sample Processing (USP) method and RRDR of rpo B gene was amplified by PCR using primers RP4T and RP8T and then sequenced by automated DNA sequencing. The nucleotide sequences of RRDR of rpo B gene were compared with the reference sequence. We observed three different types of mutation in the RRDR of rpo B gene. The frequency of mutation in codon 531 (TCG → TTG), 526 (CAC → TAC) and 516 (GAC → GTC) are 60, 26.6 and 6.6% respectively. Of the total cases studied, 6.6% cases, although resistant to rifampicin, did not show any mutation in the RRDR of rpo B gene. Codon 531 (TCG → TTG) is the most common site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-pulmonary tuberculosis followed by codon 526 (CAC → TAC) and codon 516 (GAC → GTC).