Light and nutritional regulation of transcripts encoding a wheat protein kinase homolog is mediated by cytokinins

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 7; pp. 2582 - 2586
• Main Authors: Sano, H, Youssefian, S
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 29-03-1994; National Acad Sciences; National Academy of Sciences
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; Actins; Adenine - analogs & derivatives; Adenine - pharmacology; Amino Acid Sequence; Amino acids; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Benzyl Compounds; Biological and medical sciences; CITOQUININAS; Cloning, Molecular; Complementary DNA; Culture Media - pharmacology; CYTOKININE; Cytokinins; ENFERMEDADES CARENCIALES; EXPRESION GENICA; EXPRESSION DES GENES; Food deprivation; Fundamental and applied biological sciences. Psychology; GENE; Gene expression regulation; Gene Expression Regulation - drug effects; GENES; Genes. Genome; GENETICA; GENETIQUE; Genome; Kinetin; Light; LUMIERE; LUZ; MALADIE DE CARENCE; Messenger RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Plant Growth Regulators - pharmacology; Plant Proteins; Protein-Serine-Threonine Kinases - genetics; PROTEINA QUINASA; PROTEINE KINASE; Proteins; Purines; Ribonucleic acid; RNA; RNA, Messenger - biosynthesis; SECUENCIA NUCLEICA; Seedlings; Sequence Analysis, DNA; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; Space life sciences; Triticum - genetics; Triticum - radiation effects; TRITICUM AESTIVUM; Wheat; Monocotyledones; Nucleotide sequence; Enzyme; Transferases; Gene expression; Cytokinin; Regulation(control); Gramineae; Protein kinase; Light; Angiospermae; Spermatophyta; Aminoacid sequence; Triticum aestivum
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.91.7.2582

Many metabolic processes in plants are regulated by phosphorylation of proteins by kinases, but little is known of the roles that specific protein kinases play in the various signal transduction pathways or the mechanisms by which these kinases themselves are regulated. We report here the isolation of a gene, wpk4, encoding a putative protein kinase from wheat that appears to belong to the SNF1 kinase subfamily and that shows increased transcript levels in response to multiple stimuli: light, nutrient deprivation, and cytokinin application. Although wpk4 mRNA is undetectable in etiolated seedlings, it rapidly accumulates within 1 hr of illumination. General nutrient deprivation also increases wpk4 mRNA levels, but only under light conditions. In addition, of the various phytohormones tested, cytokinin (N(6)-benzylaminopurine) specifically increases wpk4 mRNA levels regardless of the light conditions, whereas in the presence of a cytokinin antagonist the level of wpk4 mRNA is not increased by either light or nutrient deprivation. These results suggest that the light and nutrient signals that induce wpk4 mRNA accumulation may be mediated through cytokinins and provide a strong basis for examining the coordinated regulation of protein phosphorylation by light, cytokinins, and nutritional cues in a single transduction pathway