Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice

• Published in: BMC plant biology Vol. 20; no. 1; p. 90
• Main Authors: Cui, Yue, Jiang, Nan, Xu, Zhengjin, Xu, Quan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28-02-2020; BioMed Central; BMC
• Subjects: Agronomy; Biotechnology; CRISPR; CRISPR/Cas9; G proteins; Genes; Genetic aspects; Genetics; Genome editing; Genomes; Genotype & phenotype; Heterotrimeric G protein; Mutants; Mutation; Physiological aspects; Physiological regulation; Plant hardiness; Proteins; Rice; Salinity; Signal transduction; Stress tolerance; Yield components; China; Heterotrimeric G protein; Yield components; CRISPR/Cas9; Stress tolerance; Rice
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-020-2289-6

The heterotrimeric G protein complex, consisting of Gα, Gβ, and Gγ subunits, are conserved signal transduction mechanism in eukaryotes. Recent molecular researches had demonstrated that G protein signaling participates in the regulation of yield related traits. However, the effects of G protein genes on yield components and stress tolerance are not well characterized.
In this study, we generated heterotrimeric G protein mutants in rice using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology. The effects of heterotrimeric G proteins on the regulation of yield components and stress tolerance were investigated. The mutants of gs3 and dep1 generated preferable agronomic traits compared to the wild-type, whereas the mutants of rga1 showed an extreme dwarf phenotype, which led to a dramatic decrease in grain production. The mutants showed improved stress tolerance, especially under salinity treatment. We found four putative extra-large G proteins (PXLG)1-4 that also participate in the regulation of yield components and stress tolerance. A yeast two hybrid showed that the RGB1 might interact with PXLG2 but not with PXLG1, PXLG3 or PXLG4.
These findings will not only improve our understanding of the repertoire of heterotrimeric G proteins in rice but also contribute to the application of heterotrimeric G proteins in rice breeding.