Determination of Sulfonamides in Milk by Cloud Point–Salting Out Extraction and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

• Published in: Analytical Sciences Vol. 36; no. 12; pp. 1555 - 1559
• Main Authors: LI, Ze, ZHANG, Jing, YANG, Yunjia, XU, Huilan, WANG, Jianwu, YANG, Yi
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 10-12-2020; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Animals; Butanol; Chemistry; Chromatography, High Pressure Liquid; Cloud point-salting out extraction; Correlation coefficient; Correlation coefficients; Emulsification; Food Analysis - methods; High performance liquid chromatography; Hydrogen-Ion Concentration; Limit of Detection; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Milk; Milk - chemistry; Salting; Salts - chemistry; Sulfonamides; Sulfonamides - analysis; Sulfonamides - isolation & purification; Surface-Active Agents - chemistry; Tandem Mass Spectrometry; UPLC-MS/MS; Cloud point-salting out extraction; milk; UPLC-MS/MS; sulfonamides
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.20N013

A method involving cloud point–salting out extraction (CPSOE) coupled with UHPLC-MS/MS was developed for the determination of eleven sulfonamides in milk. In this study, the type and concentration of the surfactant, de-emulsification condition, pH value, volume of n-butanol, equilibration temperature and time were optimized. For this developed method, the linear range of SAs was from 0.05 to 50 μg L−1, and the correlation coefficients were higher than 0.997. The average recoveries for SAs were from 61.32 to 91.67%, and the LOQs were less than 0.06 μg kg−1.