Extensive in vivo resilience of persistent Salmonella

Chronic infections caused by persistent pathogens represent an important health problem. Here, we establish a simple practical mouse Salmonella infection model for identifying bacterial maintenance functions that are essential for persistency. In this model, a substantial fraction of Salmonella surv...

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Bibliographic Details
Published in:	PloS one Vol. 7; no. 7; p. e42007
Main Authors:	Barat, Somedutta, Steeb, Benjamin, Mazé, Alain, Bumann, Dirk
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 24-07-2012 Public Library of Science (PLoS)
Subjects:	3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - genetics Animals Antibacterial agents Antibiotics Bacteria Biofilms Biology Biosynthesis Chemotherapy Chronic infection Cloning Colony Count, Microbial Cyclopropane Disease Models, Animal E coli Escherichia coli Fatty acids Female Gallstones Genes, Bacterial - genetics Genomes Health aspects Infection Infections Kinetics Liver Liver - microbiology Liver - pathology Mathematical models Medicine Metabolism Mice Mice, Inbred BALB C Mutation Mutation - genetics Mycobacterium tuberculosis Pathogens Phenotype Salmonella Salmonella - enzymology Salmonella - genetics Salmonella - growth & development Salmonella - physiology Salmonella Infections, Animal - microbiology Salmonella Infections, Animal - pathology Salmonella Typhimurium Spleen Spleen - microbiology Spleen - pathology Tuberculosis Typhoid Switzerland
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Summary:	Chronic infections caused by persistent pathogens represent an important health problem. Here, we establish a simple practical mouse Salmonella infection model for identifying bacterial maintenance functions that are essential for persistency. In this model, a substantial fraction of Salmonella survived even several days of treatment with a potent fluoroquinolone antibiotic indicating stringency of the model. Evaluation of twelve metabolic defects revealed dramatically different requirements for Salmonella during persistency as compared to acute infections. Disrupted synthesis of unsaturated/cyclopropane fatty acids was the only defect that resulted in rapid Salmonella clearance suggesting that this pathway might contain suitable targets for antimicrobial chemotherapy of chronic infection.
Bibliography:	Conceived and designed the experiments: SB BS AM DB. Performed the experiments: SB BS AM. Analyzed the data: SB BS AM DB. Wrote the paper: SB BS DB.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0042007