Molecular Analysis of Ewing's Sarcoma: Another Fusion Gene, EWS‐E1AF, Available for Diagnosis

• Published in: Cancer science Vol. 89; no. 7; pp. 703 - 711
• Main Authors: Urano, Fumihiko, Umezawa, Akihiro, Yabe, Hiroki, Hong, Wei, Yoshida, Koichi, Fujinaga, Kei, Hata, Jun‐ichi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-07-1998; Japanese Cancer Association; John Wiley & Sons, Inc
• Subjects: Adenovirus E1A Proteins - genetics; Adolescent; Adult; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Child; Child, Preschool; Chimeras; Diagnosis; Diseases of the osteoarticular system; DNA sequencing; DNA, Complementary - chemistry; E1AF; Ets; Ewing's sarcoma; EWS protein; Female; Fusion protein; Gene Rearrangement; Genes; Genetic analysis; Heterogeneous-Nuclear Ribonucleoproteins; Humans; key words; Male; Medical sciences; Molecular diagnosis; Molecular Sequence Data; Oncogene Proteins, Fusion - genetics; Phenotypes; Polymerase Chain Reaction; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins c-ets; Ribonucleic acid; Ribonucleoproteins - genetics; RNA; RNA binding protein; RNA-Binding Protein EWS; Sarcoma, Ewing - diagnosis; Sarcoma, Ewing - genetics; Sarcoma, Ewing - pathology; Tumor Cells, Cultured; Tumorigenesis; Tumors; Tumors of striated muscle and skeleton; Young adults; Human; Immunohistochemistry; Correlation; Ewing sarcoma; Diseases of the osteoarticular system; Malignant tumor; Gene expression; Genetic determinism; Phenotype; Gene; Adult; Bone; Molecular biology; Child; Chimera
• ISSN: 0910-5050; 1347-9032; 1349-7006; 1876-4673
• DOI: 10.1111/j.1349-7006.1998.tb03274.x

Ewing's sarcoma, one of the most malignant tumors of children and young adults, expresses specific chimeric genes, e.g. EWS‐FLI‐1, EWS‐ERG, EWS‐ETV1 and EWS‐FEV. In this paper, we extensively characterized a new fusion gene, EWS‐E1AF by means of whole cDNA sequencing, RNA blot analysis, DNA blot analysis and chromosomal analysis, and showed it to be available for the diagnosis of Ewing's sarcoma and to participate in the oncogenesis of Ewing's sarcoma. Furthermore, we conducted a genetic analysis of Ewing family tumors in conjuction with immunohistochemical analysis and ultrastructural analysis. Our results demonstrate some limitations of both genetic analysis and histopathological analysis, and establish the relationship between neurogenic phenotypes and chimera genes.