Associations between autoantibodies against apolipoprotein B-100 peptides and vascular complications in patients with type 2 diabetes

• Published in: Diabetologia Vol. 52; no. 7; pp. 1426 - 1433
• Main Authors: Fredrikson, G. N, Anand, D. V, Hopkins, D, Corder, R, Alm, R, Bengtsson, E, Shah, P. K, Lahiri, A, Nilsson, J
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-07-2009; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Adult; Albuminuria - epidemiology; Albuminuria - immunology; Amino acids; Antigens; Apolipoprotein B-100 - immunology; Apolipoproteins; Atherosclerosis; Autoantibodies - blood; Biological and medical sciences; Biomarkers; Biomarkers - blood; Calcification; Cardiovascular disease; Clinical Medicine; Coronary Disease - epidemiology; Coronary Disease - immunology; Coronary vessels; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction; Creatinine; Diabetes; Diabetes Complications - epidemiology; Diabetes Complications - immunology; Diabetes Mellitus, Type 2 - epidemiology; Diabetes Mellitus, Type 2 - immunology; Diabetes. Impaired glucose tolerance; Diabetic Angiopathies - epidemiology; Diabetic Angiopathies - immunology; Diabetic Nephropathies - epidemiology; Diabetic Nephropathies - immunology; Diabetic Retinopathy - epidemiology; Diabetic Retinopathy - immunology; Endocrine pancreas. Apud cells (diseases); Endocrinology and Diabetes; Endocrinopathies; Endokrinologi och diabetes; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Hospitals; Human Physiology; Humans; Hypotheses; Immunoglobulin G - blood; Internal Medicine; Klinisk medicin; Lipoproteins, LDL - immunology; Male; Medical and Health Sciences; Medical sciences; Medicin och hälsovetenskap; Medicine; Medicine & Public Health; Metabolic Diseases; Microcirculation - immunology; Middle Aged; Nervous system (semeiology, syndromes); Neurology; Ophthalmology; Oxidation; Oxidative stress; Peptides; Peripheral neuropathy; Retinopathies; Risk Factors; Scintigraphy; Urine; Veins & arteries; United Kingdom > UK; Los Angeles California; United States > US; Low-density lipoprotein; Antibodies; Oxidation; Retinopathy; Peripheral neuropathy; Endocrinopathy; Autoimmunity; Type 2 diabetes; Human; Nervous system diseases; Peptides; Antibody; Autoantibody; Metabolic diseases; Apolipoprotein B; Lipoprotein LDL; Eye disease; Association; Complication; Peripheral nerve disease
• ISSN: 0012-186X; 1432-0428; 1432-0428
• DOI: 10.1007/s00125-009-1377-9

Aims/hypothesis Oxidation of LDL in the arterial extracellular matrix is a key event in the development of atherosclerosis and autoantibodies against oxidised LDL antigens reflect disease severity and the risk of developing acute cardiovascular events. Since type 2 diabetes is associated with increased oxidative stress, we tested the hypothesis that autoantibodies against oxidised LDL antigens are biomarkers for vascular complications in diabetes. Methods We studied 497 patients with type 2 diabetes without clinical signs of coronary heart disease. Oxidised LDL autoantibodies were determined by ELISA detecting IgG and IgM specific for native and malondialdehyde (MDA)-modified apolipoprotein B-100 peptides p45 and p210. The severity of coronary disease was assessed as the coronary artery calcium score. Results Patients affected by retinopathy had significantly higher levels of IgG against MDA-p45 and MDA-p210. In contrast, high levels of autoantibodies against the corresponding native peptides were associated with less coronary calcification and a lower risk of progression of coronary disease. Conclusions/interpretation Our observations suggest that LDL oxidation is involved in the pathogenesis of diabetic retinopathy and that autoantibodies against apolipoprotein B peptides may act as biomarkers for both micro- and macrovascular complications in diabetes.