Validation of an LDH assay for assessing nanoparticle toxicity

• Published in: Toxicology (Amsterdam) Vol. 287; no. 1; pp. 99 - 104
• Main Authors: Han, Xianglu, Gelein, Robert, Corson, Nancy, Wade-Mercer, Pamela, Jiang, Jingkun, Biswas, Pratim, Finkelstein, Jacob N, Elder, Alison, Oberdörster, Günter
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ireland Ltd 05-09-2011; Elsevier
• Subjects: Adenine; Animals; Biological and medical sciences; carbon; Cells, Cultured; copper; Copper - toxicity; Cytotoxicity; Dissolution assay; Dose-Response Relationship, Drug; Emergency; L-Lactate Dehydrogenase - metabolism; lactate dehydrogenase; LDH assay; Male; Medical sciences; NAD (coenzyme); NAD - metabolism; NADH; Nanoparticle; nanoparticles; Nanoparticles - toxicity; Particle Size; Rats; silver; Silver - toxicity; Titanium - toxicity; Titanium dioxide; Toxicology; LDH assay; Cytotoxicity; NADH; Nanoparticle; Titanium dioxide; Dissolution assay; Validation; Toxicity; Ultrafine particle; Dissolution
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/j.tox.2011.06.011

Highlights ► We examined if some nanoparticles (NPs) can interfere with an LDH assay. ► Copper NP and a silver NP could interfere with the assay by inactivating LDH. ► Titanium dioxide NPs were found to adsorb LDH but only at high doses. ► Thus, the validity of the assay for assessing NP toxicity depends on NP types.