Epithelial-Mesenchymal Transition (EMT) Gene Variants and Epithelial Ovarian Cancer (EOC) Risk

• Published in: Genetic epidemiology Vol. 39; no. 8; pp. 689 - 697
• Main Authors: Lin, Hui-Yi, Tyrer, Jonathan P., Lawrenson, Kate, Dennis, Joe, Anton-Culver, Hoda, Bean, Yukie T., Bisogna, Maria, Brinton, Louise A., Brooks-Wilson, Angela, Carty, Karen, Chen, Zhihua, Chen, Y. Ann, Cook, Linda S., Cramer, Daniel W., Cunningham, Julie M., Dansonka-Mieszkowska, Agnieszka, du Bois, Andreas, Despierre, Evelyn, Doherty, Jennifer A., Dörk, Thilo, Easton, Douglas F., Eccles, Diana M., Fasching, Peter A., Fridley, Brooke L., Gao, Yu-Tang, Gentry-Maharaj, Aleksandra, Giles, Graham G., Glasspool, Rosalind, Gronwald, Jacek, Harter, Philipp, Hasmad, Hanis N., Hein, Alexander, Heitz, Florian, Hildebrandt, Michelle A. T., Hogdall, Claus K., Hogdall, Estrid, Iversen, Edwin S., Jakubowska, Anna, Ji, Bu-Tian, Karlan, Beth Y., Jim, Heather, Kiemeney, Lambertus A., Kjaer, Susanne K., Le, Nhu D., Lee, Alice W., Lester, Jenny, Levine, Douglas A., Liang, Dong, Lim, Boon Kiong, Lissowska, Jolanta, Lubinski, Jan, Lundvall, Lene, Matsuo, Keitaro, McGuire, Valerie, Menon, Usha, Milne, Roger L., Modugno, Francesmary, Moysich, Kirsten B., Nevanlinna, Heli, Eilber, Ursula, Olson, Sara H., Orlow, Irene, Orsulic, Sandra, Pearce, Celeste L., Pejovic, Tanja, Permuth-Wey, Jennifer, Pike, Malcolm C., Poole, Elizabeth M., Risch, Harvey A., Rosen, Barry, Rudolph, Anja, Runnebaum, Ingo B., Rzepecka, Iwona K., Salvesen, Helga B., Schwaab, Ira, Shu, Xiao-Ou, Shvetsov, Yurii B., Song, Honglin, Southey, Melissa C., Spiewankiewicz, Beata, Terry, Kathryn L., Thompson, Pamela J., Tangen, Ingvild L., Tworoger, Shelley S., Vergote, Ignace, Wang-Gohrke, Shan, Wicklund, Kristine G., Wu, Anna H., Wu, Xifeng, Woo, Yin-Ling, Yang, Hannah, Zheng, Wei, Ziogas, Argyrios, Kelemen, Linda E., Berchuck, Andrew, Monteiro, Alvaro N. A., Gayther, Simon A., Narod, Steven A., Pharoah, Paul D. P., Sellers, Thomas A.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-12-2015; Wiley Subscription Services, Inc
• Subjects: Adult; Aged; Carcinoma, Ovarian Epithelial; epithelial-mesenchymal transition; Epithelial-Mesenchymal Transition - genetics; Female; Genes; Genetic Predisposition to Disease; Genome-Wide Association Study; Genotype; Humans; Middle Aged; Neoplasms, Glandular and Epithelial - epidemiology; Neoplasms, Glandular and Epithelial - genetics; Odds Ratio; Ovarian cancer; Ovarian Neoplasms - epidemiology; Ovarian Neoplasms - genetics; Polymorphism, Single Nucleotide - genetics; Risk; single-nucleotide polymorphisms; White People; ovarian cancer; single-nucleotide polymorphisms; epithelial-mesenchymal transition
• ISSN: 0741-0395; 1098-2272; 1098-2272
• DOI: 10.1002/gepi.21921

ABSTRACT
Epithelial‐mesenchymal transition (EMT) is a process whereby epithelial cells assume mesenchymal characteristics to facilitate cancer metastasis. However, EMT also contributes to the initiation and development of primary tumors. Prior studies that explored the hypothesis that EMT gene variants contribute to epithelial ovarian carcinoma (EOC) risk have been based on small sample sizes and none have sought replication in an independent population. We screened 15,816 single‐nucleotide polymorphisms (SNPs) in 296 genes in a discovery phase using data from a genome‐wide association study of EOC among women of European ancestry (1,947 cases and 2,009 controls) and identified 793 variants in 278 EMT‐related genes that were nominally (P < 0.05) associated with invasive EOC. These SNPs were then genotyped in a larger study of 14,525 invasive‐cancer patients and 23,447 controls. A P‐value <0.05 and a false discovery rate (FDR) <0.2 were considered statistically significant. In the larger dataset, GPC6/GPC5 rs17702471 was associated with the endometrioid subtype among Caucasians (odds ratio (OR) = 1.16, 95% CI = 1.07–1.25, P = 0.0003, FDR = 0.19), whereas F8 rs7053448 (OR = 1.69, 95% CI = 1.27–2.24, P = 0.0003, FDR = 0.12), F8 rs7058826 (OR = 1.69, 95% CI = 1.27–2.24, P = 0.0003, FDR = 0.12), and CAPN13 rs1983383 (OR = 0.79, 95% CI = 0.69–0.90, P = 0.0005, FDR = 0.12) were associated with combined invasive EOC among Asians. In silico functional analyses revealed that GPC6/GPC5 rs17702471 coincided with DNA regulatory elements. These results suggest that EMT gene variants do not appear to play a significant role in the susceptibility to EOC.