First isolation and genotyping of Bartonella henselae from a cat living with a patient with cat scratch disease in Southeast Europe

• Published in: BMC infectious diseases Vol. 19; no. 1; p. 299
• Main Authors: Stepanić, Maja, Duvnjak, Sanja, Reil, Irena, Špičić, Silvio, Kompes, Gordan, Beck, Relja
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02-04-2019; BioMed Central; BMC
• Subjects: Animal genetic engineering; Antibodies; Azithromycin; Bacteremia; Bartonella; Bartonella henselae; Blood; Case Report; Cat; Cat scratch disease; Causes of; Child; Culture; Deoxyribonucleic acid; Diagnosis; Disease; DNA; Doxycycline; EDTA; Genotypes; Genotyping; Health aspects; Health care; Health risks; Identification and classification; Immunoglobulin G; Immunoglobulins; Infections; Infectious diseases; Knowledge; Lymphadenopathy; Lymphatic diseases; Lymphatic system; Nucleic acids; Pain; Pathogens; Patients; Polymerase chain reaction; Public health; rRNA 16S; Ultrasonic imaging; Zoonoses; Croatia; South East Europe; Europe; Genotyping; MLST; Cat; Bartonella henselae; Cat-scratch disease; Child; Culture; Croatia
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-019-3929-z

The bacterial genus Bartonella is distributed worldwide and poses a public health risk. Cat-scratch disease caused by B. henselae in Croatia was first described in 1957. It is present throughout the country: a survey of serum samples from 268 Croatian patients with lymphadenopathy showed that 37.7% had IgG antibodies. Despite this prevalence, we are unaware of reports of Bartonella culturing from infected humans or cats in Croatia or elsewhere in southeast Europe.
Here we describe the diagnosis of a 12-year-old child with lymphadenopathy in Croatia with cat-scratch disease based on antibody detection and clinical signs, and the subsequent culturing and genotyping of B.henselae from the cat's blood. The B. henselae isolate was grown on different blood agar plates and its identity was confirmed based on polymerase chain reaction (PCR) amplification of 16S ribosomal deoxyribonucleic acid (16S rDNA) and sequencing. Multi-locus sequence typing (MLST) identified the strain genotype as sequence type 5, commonly found zoonotic B. henselae strain in cats. The child recovered after azithromycin therapy, and B. henselae in the cat was eliminated within three months after doxycycline treatment.
This is, to our knowledge, the first report of B. henselae culturing and MLST-based genotyping from cat's blood in southeast Europe. Our ability to detect B. henselae in blood through culturing but not PCR suggests that the prevalence of infected cats with low bacteremia is very high, suggesting the need to develop faster, more sensitive detection assays.